福建农业学报2026，Vol.41Issue(2)：148-158,11.DOI:10.19303/j.issn.1008-0384.2026.02.002
铁皮石斛DobHLH63基因的克隆及表达分析
Cloning and Expression of DobHLH63 Gene in Dendrobium officinale
摘要
Abstract
[Objective]Structure,function,and expression of the transcription factor in the eukaryotes crucial in plant growth,development,and stress response of the basic helix loop helix(bHLH)proteins in Dendrobium officinale were analyzed.[Methods]DobHLH63 was cloned using RT-PCR and bioinformatically analyzed.Spatiotemporal expressions of the gene under various plant growth regulators,including abscisic acid,salicylic acid and methyl jasmonate,as well as abiotic stresses,such as drought and salt-alkali stress,were determined by qRT-PCR.[Results]The coding region of DobHLH63 was 966 bp long and encoded 321 amino acids.The gene was possibly located in the nucleus and cytoplasm with 29 phosphorylation sites but had no apparent signal peptide or transmembrane domain.It closely related to and shared functions with PzbHLH63 and paralog DobHLH74 of Aponogeton madagascariensis.The WD40 transcription factor appeared to be an important interacting protein.The higher expression in the leaves than in the stems responded to abiotic stress and hormone treatments,especially alkaline salt stress and salicylic acid treatment.[Conclusion]DobHLH63 regulated the response of D.officinale to abiotic stress and hormones by binding to the transcription factors,such as WD40.关键词
铁皮石斛/DobHLH63转录因子/基因克隆/基因表达Key words
Dendrobium officinale/bHLH63 transcription factor/gene cloning/gene expression分类
农业科技引用本文复制引用
王欣睿,于莹,陈宏宇,杨奕樱,李娟,骆文慧..铁皮石斛DobHLH63基因的克隆及表达分析[J].福建农业学报,2026,41(2):148-158,11.基金项目
贵州省科技厅科技计划项目(黔科合基础-ZK[2024]一般353) （黔科合基础-ZK[2024]一般353）
贵州省中医药管理局中医药、民族医药科学技术课题(QZYY-2024-115) （QZYY-2024-115）
