福建农业学报2026，Vol.41Issue(2)：190-197,8.DOI:10.19303/j.issn.1008-0384.2026.02.006
油樟中呋喃型木脂素对层出镰刀菌的抑制活性及作用机理
Inhibitory Activity of Cinnamomum longepaniculatum Lignans on Growth of Fusarium proliferatum
摘要
Abstract
[Objective]Antifungal activity of Tetrahydrofuranoid lignans from Cinnamomum longepaniculatum on Fusarium proliferatum was investigated for utilization of the natural resource.[Methods]Inhibitory effects of C.longepaniculatum lignans on spore germination and mycelial growth of F.proliferatum was tested to determine the structure-activity relationship(SAR)between the molecule and its biological activity.Subsequently,effects of the active chemical components on the mycelial morphology,dry weight,cell membrane conductivity,protein content,malondialdehyde(MDA),and reducing sugars as well as protective enzyme activities of the fungus were analyzed to understand the inhibition mechanism.[Results]The tetrahydrofuran-type lignans of C.longepaniculatum exhibited potent antifungal activity on F.proliferatum.At the dosage of 100 µg·mL-1,it inhibited 16.7%-75.2%mycelial growth of the fungus.The active(+)-piperitol in the lignans showed an EC50 of 38.615 µg·mL-1.Under the treatment,significantly,the fungal mycelia was morphologically altered,dry weight decreased,cell relative membrane conductivity raised,intracellular protein content declined,MDA increased,reducing sugar lowered,and activities of multiple protective enzyme systems reduced.[Conclusion]The furan-type lignans from C.longepaniculatum displayed a potent in vitro antifungal activity on F.proliferatum.The efficacy appeared to closely correlate to its chemical structure,as indicated by the SAR analysis.The functions of mycelial cell membranes of the fungus were disrupted and the protective enzyme activities downregulating by the(+)-piperitol treatments resulting in inhibition of spore germination and retardation of mycelial development of the pathogen.关键词
油樟/层出镰刀菌/抑菌活性/(+)-piperitolKey words
Cinnamomum longepaniculatum/Fusarium proliferatum/antibacterial activity/(+)-piperitol分类
农业科技引用本文复制引用
徐孟飞,李欣瑜,程家豪,李明瑞,陈景新,邱远望,倪林..油樟中呋喃型木脂素对层出镰刀菌的抑制活性及作用机理[J].福建农业学报,2026,41(2):190-197,8.基金项目
福建省林业科技项目(2023FKJ28) （2023FKJ28）
中央财政林业科技推广项目(闽[2022]TG16号) （闽[2022]TG16号）
福建省科技计划农业引导性(重点)项目(2023N0003) （重点）
