福建农业学报2026，Vol.41Issue(3)：338-346,9.DOI:10.19303/j.issn.1008-0384.2026.03.007
响应面法优化猪肚菇液体菌种发酵配方
Response Surface-optimized Formulation for Culturing Pleurotus giganteus Spawns
摘要
Abstract
[Objective]Culture medium for growing Pleurotus giganteus spawns was formulated by means of response surface optimization.[Methods]A single factor fermentation experiment was carried out in triangular shaking flasks to evaluate the effects of 6 carbon sources,8 nitrogen sources,and 11 inorganic salts on mycelial growth of P.giganteus ZD9.Box-Behnken response surface design was used to optimize the medium formula.[Results]The dry weight biomass of the spawns peaked at 13.30 g·L-1 on the 6th day of culture in the optimized medium,which was formulated with the following ingredients at per liter rates of 70 g whole wheat flour,27 g soybean meal powder,2 g KCl,and 2 g MgSO4·7H2O to obtain a mycelial biomass at the concentration of 25.06 g·L-1.[Conclusion]The optimized medium for culturing P.giganteus spawns achieved an 88.42%improvement on mycelial biomass production.关键词
猪肚菇/液体菌种/菌丝生物量/响应面法Key words
Pleurotus giganteus/mushroom spawns/mycelium biomass/response surface methodology分类
农业科技引用本文复制引用
郑泽杰,施俊玲,杨睿,黄志龙,赖淑芳,胡开辉,金文松,蔡立君..响应面法优化猪肚菇液体菌种发酵配方[J].福建农业学报,2026,41(3):338-346,9.基金项目
福建省科技厅对外合作项目(2022I0009) （2022I0009）
福建省中央引导地方科技发展资金项目(2022L3005) （2022L3005）
福建农林大学科技创新专项基金项目(KFB23070A) （KFB23070A）
