南方农业学报2026，Vol.57Issue(4)：1027-1037,11.DOI:10.3969/j.issn.2095-1191.2026.04.007
60Co-γ射线辐射对橡胶草种子萌发、幼苗生长及生理特性的影响
Effects of 60Co-γ radiation on seed germination,seedling growth,and physiological characteristics of Taraxacum kok-saghyz Rodin
摘要
Abstract
[Objective]This study aimed to investigate the effects of 60Co-γ radiation mutagenesis on seed germina-tion,seedling growth,and physiological characteristics of Taraxacum kok-saghyz Rodin,to determine the optimal radia-tion dose,providing theoretical basis for breeding novel superior varieties and enriching Taraxacum kok-saghyz Rodin germplasm resources.[Method]Seeds of Taraxacum kok-saghyz Rodin K445 were used as experimental materials.Seeds were treated with different 60Co-γ radiation doses[0(control,CK),60,100,140,180 Gy]to conduct comparative analysis of the effects of different radiation dose treatments on seed germination indicators,seedling morphological indi-cators,and physiological indicators of Taraxacum kok-saghyz Rodin.[Result]After different doses of 60Co-γ radiation,the germination rate,germination potential,germination index,and seedling emergence rate of Taraxacum kok-saghyz Rodin were significantly decreased compared to control group(P<0.05,the same below),exhibiting a gradual decreasing trend with increasing radiation dose,indicating that seed germination was markedly inhibited.After different doses of 60Co-γ radiation for Taraxacum kok-saghyz Rodin seeds,seedling growth was markedly inhibited,with marked morpho-logical radicle differences between different dose groups;compared with the control group,radicles were noticeably shortened after radiation,with brown necrosis at the root tips,and radicle necrosis turned severer as radiation dose increased.As radiation dose increased,both seedling emergence rate and relative seedling rate of Taraxacum kok-saghyz Rodin seeds showed a decreasing trend,with significant differences between dose treatment groups;leaf length,root length,dry weight,and fresh weight of seedlings also generally decreased,indicating that different 60Co-γ radiation doses markedly inhibited seedling growth of Taraxacum kok-saghyz Rodin,and seedling growth inhibition was intensified as radiation dose increased.By establishing the optimal regression equation of radiation dose(x)and relative seedling rate(y)as y=-0.550x+84.825,R2=0.828,the semi-lethal dose(LD50)for 50%relative seedling rate of Taraxacum kok-saghyz Rodin was calculated to be 63.32 Gy,which was the recommended radiation dose.As radiation dose increased,contents of hydrogen peroxide(H2O2)and malondialdehyde(MDA)first increased and then decreased,all significantly higher than those in the control group;activities of peroxidase(POD),superoxide dismutase(SOD),and catalase(CAT)in seedling leaves showed an initial increase followed by a decrease,all significantly higher than the control group.[Conclusion]Taraxacum kok-saghyz Rodin exhibits high sensitivity to 60Co-γ radiation,with an LD50 of only 63.32 Gy.Under 60Co-γ radiation,the seed germination and seedling growth of Taraxacum kok-saghyz Rodin show dose dependency and inhibitory effects.Appropriate radiation dose can activate antioxidant defense system in Taraxacum kok-saghyz Rodin seedlings,but excessive doses can damage the defense system,causing increased lipid peroxidation of cell membranes.关键词
橡胶草/60Co-γ射线辐射/种子萌发/幼苗生长/半致死剂量(LD50)Key words
Taraxacum kok-saghyz Rodin/60Co-γ radiation/seed germination/seedling growth/semi-lethal dose(LD50)分类
农业科技引用本文复制引用
郑立鹏,高强,徐麟,严青青,曹佳敏,赵晶,张龑,吐汗姑丽·托合提..60Co-γ射线辐射对橡胶草种子萌发、幼苗生长及生理特性的影响[J].南方农业学报,2026,57(4):1027-1037,11.基金项目
新疆天山英才培养计划项目(2023TSYCLJ0010) （2023TSYCLJ0010）
新疆"三农"骨干人才培养项目(2024SNGGNT071) （2024SNGGNT071）
新疆农业科学院科技稳定支持项目(xjnkywdzc-2025001-22) Xinjiang Tianshan Talent Cultivation Project(2023TSYCLJ0010) （xjnkywdzc-2025001-22）
Xinjiang Core Personnel Cul-tivation Project for Agriculture,Rural Areas,and Farmers(2024SNGGNT071) （2024SNGGNT071）
Stably Supported Science and Techno-logy Innovation Project of Xinjiang Academy of Agricultural Sciences(xjnkywdzc-2025001-22) （xjnkywdzc-2025001-22）
