贵州中医药大学学报2026，Vol.48Issue(3)：35-40,6.DOI:10.16588/j.cnki.issn2096-8426.2026.03.007
黄连解毒汤调节2型糖尿病大鼠粪便乳酸杆菌的实验研究
Investigation on the Effect of Huanglian Jiedu Decoction on Fecal Lactobacillus in Type 2 Diabetic Rats
摘要
Abstract
Objective To investigate the effect of Huanglian Jiedu Decoction on the quantity of Lactobacillus in type 2 diabetes mellitus(T2DM)model rats by fluorescence quantitative PRC(qPCR).Methods 60 SD rats were randomly divided into a blank group(10 rats)and a model group(50 rats).After 7 days of adaptive feeding,the rats in the model group underwent induction with a high-fat diet combined with streptozotocin(STZ)injection to establish the diabetes model.Fasting blood glucose≥16.7 mmol/L after modeling was regar-ded as the criterion for successful model establishment.The successfully modeled rats were further divided into five groups by the random number table method,with 10 rats in each group:a model group,a high-dose Huan-glian Jiedu Decoction group(6.30 g·kg-1),a medium-dose Huanglian Jiedu Decoction group(3.15 g·kg-1),a low-dose Huanglian Jiedu Decoction group(1.58 g·kg-1),and a control group(metformin,200 mg·kg-1).From the second day after modeling,rats in each Huanglian Jiedu Decoction group and the control group were given the corresponding drugs by intragastric administration for consecutive 21 days.Rats in the blank group and the model group were given an equal volume of normal saline by intragastric administration synchro-nously.After the completion of drug intervention,all rats were fasted for 12 hours with free access to drinking water.Blood samples were collected via tail vein puncture to detect fasting blood glucose.Subsequently,fresh feces were collected for DNA extraction and qPCR detection.Results The abundance of Lactobacillus in the model group was significantly lower than that in the blank group.After intervention with Huanglian Jiedu Decoc-tion and metformin,the abundance of Lactobacillus was markedly restored.Compared with the model group,both the low dose Huanglian Jiedu Decoction group and the control group showed a significant increase in Lacto-bacillus abundance(P<0.05).Conclusion Huanglian Jiedu Decoction can effectively increase the intestinal abundance of Lactobacillus in diabetic rats,and the low-dose intervention exhibits the most prominent therapeu-tic effect.关键词
2型糖尿病/乳酸杆菌/黄连解毒汤/荧光定量PCRKey words
type 2 diabetes/Lactobacillus/Huanglian Jiedu Decoction/fluorescence quantitative PCR分类
医药卫生引用本文复制引用
奚锦,王巍,王照朋,吴晨曦,王明英,彭智应玲,焦厚佳,王巧,何光志..黄连解毒汤调节2型糖尿病大鼠粪便乳酸杆菌的实验研究[J].贵州中医药大学学报,2026,48(3):35-40,6.基金项目
1.贵州省中医药管理局中医药、民族医药科学技术研究项目(QZYY-2022-005) （QZYY-2022-005）
2.贵州省科学技术基金项目(黔科合基础[2020]1Y392) （黔科合基础[2020]1Y392）
3.大学生创新创业项目(S202410662147) （S202410662147）
4.贵州中医药大学科研项目(贵中医科余2024703). （贵中医科余2024703）
