贵州农业科学2026，Vol.54Issue(5)：12-21,10.DOI:10.3969/j.issn.1001-3601.2026.05.002
芭蕉根内生细菌多样性及其对宿主代谢产物的影响
Diversity of Endophytic Bacteria and Their Effect on Metabolites of Rhizoma Musae
摘要
Abstract
[Objective]The community composition and diversity characteristics of endophytic bacteria in Rhizoma Musae were explored and the correlations between endophytic bacteria and active ingredients in Rhizoma Musae were analyzed to provide the scientific basis for improving quality of Rhizoma Musae.[Method]The endophytic bacterial communities in Rhizoma Musae and metabolic products of the medicinal materials were analyzed by 16S rDNA high-throughput sequencing and liquid chromatography-mass spectrometry(LC-MS)technique.The endophytic bacterial groups closely related with metabolic products and active ingredients of Rhizoma Musae were screened by Pearson correlation analysis.[Result]At phylum level,the relative abundance of Mycoplasmatota,Pseudomonadota and Actinomycetota was 63.42％-86.59％,7.84％-31.52％and 1.02％-2.63％respectively.At genus level,Candidatus Phytoplasma was a dominant species and its relative abundance reached 63.42％-86.59％.117 mutual differential metabolites(DEMs)including glycoside,glycolipids,polyketone,terpene,sterides and so on were screened from Rhizoma Musae samples collected from 5 Rhizoma Musae producing areas and mainly enriched in metabolic pathways of ABC transport protein,flavonoid biosynthesis,amino acid biosynthesis.The correlation analysis showed that Inquilinus was significantly and positively related to total phenolic components,and significantly and negatively related to flavonoids compounds.Catenulispora and Mycobacterium were significantly and positively related to total alkaloids components.[Conclusion]The community structure and diversity of endophytic bacteria in Rhizoma Musae from different producing areas are relatively stable.There are differences in the relative abundance of the dominant endophytic bacteria.There are significant correlations between bacterial genera of endophytic bacteria and metabolites of Rhizoma Musae.The specific bacterial genera may involve in synthesis or conversion process of active ingredients by means of regulating key metabolic pathway.The synthesis of secondary metabolites such as flavone and alkaloid in Rhizoma Musae may be closely related to endophytic bacteria.关键词
芭蕉根/内生细菌/代谢组学/活性成分/药材品质Key words
Rhizoma Musae/endophytic bacteria/metabolomics/active ingredients/quality of medicinal material分类
农业科技引用本文复制引用
张剑,熊新颖,杨亚娥,王忠泽,杨成龙,杨小雨,雷静,龙佩霖..芭蕉根内生细菌多样性及其对宿主代谢产物的影响[J].贵州农业科学,2026,54(5):12-21,10.基金项目
贵州省农业科学院一般基金项目(黔农科一般基金[2024]10)(黔农科一般基金[2025]54) （黔农科一般基金[2024]10）
贵州省科技计划项目(黔科合成果[2024]一般091) （黔科合成果[2024]一般091）
贵州省育种科研基础平台创新能力建设项目(黔科合服企[2022]014) （黔科合服企[2022]014）
