贵州农业科学2026，Vol.54Issue(5)：39-46,8.DOI:10.3969/j.issn.1001-3601.2026.05.005
转Fa6-SFT基因菊苣的抗逆性研究
Study on Stress Resistance of Cichorium intybus Transformed with Fa6-SFT Gene
摘要
Abstract
[Objective]The regulatory function of Fa6-SFT gene in drought and high temperature resistance of Cichorium intybus was investigated to provide a theoretical basis for resistance improvement of chicory and other pasture varieties.[Method]The structured overexpression vector of Fa6-SFT gene was transformed into C.intybus by the agrobacterium-mediated method.The expression level of Fa6-SFT gene in transgenic C.intybus was analyzed by using quantitative real-time PCR.The physiological and biochemical indexes including enzyme activity,chlorophyll,MDA,cellulose,starch and soluble sugar content of transgenic C.intybus plants under drought and high-temperature stress were determined to analyze the resistance.[Result]The Fa6-SFT expression of nine successfully obtained transgenic C.intybus plants after drought and high-temperature treatment was significantly upregulated.The chlorophyll content of transgenic C.intybus plants under drought and high-temperature stress was significantly higher than that of wild C.intybus plants.The enzyme activity,and cellulose,and starch content of transgenic C.intybus plants under drought and high-temperature stress increased but its MDA content reduced significantly.[Conclusion]The overexpression of Fa6-SFT gene can improve drought and high-temperature resistance of transgenic C.intybus plants significantly.Therefore,it is speculated that Fa6-SFT gene is closely associated with regulation and control of drought and high-temperature resistance in plants.关键词
Fa6-SFT基因/转基因/载体构建/遗传转化/干旱胁迫/高温胁迫/生理生化/抗逆性Key words
Fa6-SFT gene/transgenosis/vector construction/genetic transformation/drought stress/high-tempe-rature stress/physiology and biochemistry/stress resistance分类
农业科技引用本文复制引用
陈锡,王小利,赵德刚..转Fa6-SFT基因菊苣的抗逆性研究[J].贵州农业科学,2026,54(5):39-46,8.基金项目
贵州省科技计划项目(黔科合基础-ZK[2022]一般222) （黔科合基础-ZK[2022]一般222）
贵州省农业科学院开放基金项目(黔农科开放基金[2024]01号) （黔农科开放基金[2024]01号）
