海南大学学报（自然科学版中英文）2026，Vol.44Issue(2)：161-173,13.DOI:10.65658/j.hndk.2025052202
水产病原弧菌拮抗菌的筛选及其抗菌物质分离
Screening of antagonistic bacteria against aquatic pathogenic Vibrio and isolation of their antibacterial substances
摘要
Abstract
In order to explore the optimal fermentation conditions and stability of the antibacterial substances in the supernatant,the plate confrontation method was used to screen antagonistic bacteria with the significant antagonistic effects against Vibrio harveyi,and the salting method with the different ammonium sulfate saturation was performed to extract the crude antibacterial proteins.The results showed that Bacillus velezensis BS2-7 had the most significant antagonistic effects against the aquaculture pathogen Vibrio harveyi GDH11385,the fermentation medium for Bacillus velezensis and fermentation temperature had no significant effects on the antibacterial activity,while the fermentation duration had a significant effect(P<0.05);the supernatant had a wide antibacterial spectrum and showed the antagonistic activity against Vibrio harveyi,Vibrio owensii,Vibrio parahaemolyticus,and Bacillus altitudinis;the temperature,pH,and ultraviolet irradiation had significant effects(P<0.05);when the ammonium sulfate with 10%saturation and 20%saturation were added into the supernatant,the crude proteins separated from the fractions had the antibacterial activity against Vibrio harveyi and Vibrio owensii.Our findings provide the sample support and the theoretical basis for the development of antibacterial protein drugs against Vibrio pathogens.关键词
哈维氏弧菌/欧文氏弧菌/贝莱斯芽孢杆菌/抗菌蛋白Key words
Vibrio harveyi/Vibrio owensii/Bacillus velezensis/antibacterial protein分类
社会科学引用本文复制引用
叶绵权,王欣怡,池梓榕,蔡晓霓,龙昊,谢珍玉,任伟..水产病原弧菌拮抗菌的筛选及其抗菌物质分离[J].海南大学学报（自然科学版中英文）,2026,44(2):161-173,13.基金项目
本文得到国家自然科学基金项目(42306140)、海南省科技人才创新项目(KJRC2023C40)和海南省虾产业技术体系专项资金项目(HNARS-10-ZJ03)的支持. This work was supported by the National Natural Science Foundation of China(42306140),the Hainan Province Science and Technology Talent Innovation Project(KJRC2023C40),and the Special Fund Project of the Hainan Province Shrimp Industry Technology System(HNARS-10-ZJ03). （42306140）
