海南医科大学学报2026，Vol.32Issue(10)：721-729,9.DOI:10.13210/j.cnki.jhmu.20250530.001
吡非尼酮对三阴性乳腺癌4T1细胞增殖、迁移和侵袭影响的机制研究
Effects of pirfenidone on the proliferation,migration,and invasion of triple-negative breast cancer 4T1 cells
摘要
Abstract
Objective:To explore the effects of of pirfenidone(PFD)on the proliferation,migration,and invasion abilities of triple-negative breast cancer 4T1 cells and its mechanisms.Methods:The triple-negative breast cancer 4T1 cell line was cultured.The 4T1 cells treated with different concentrations of PFD(2,4,6,and 8 mmol/L)were taken as the drug treatment group,and the 4T1 cells not treated with PFD were taken as the control group.The expression of arylamine N-acetyltransferase 1(NAT1)protein was detected by Western blot.The effect of PFD on 4T1 cells' proliferation ability was detected by CCK-8 assay and clone formation assay.The scratch assay and Transwell migration assay were used to detect the effect of PFD on 4T1 cells' migration ability.The Transwell invasion assay was used to detect the effect of PFD on 4T1 cells' invasion ability.Molecular docking was used to verify the interaction between PFD and NAT1.Results:Compared to the control group,PFD significantly inhibited the protein expression of NAT1 at the concentration of 8 mmol/L(P<0.000 1);Compared to the control group,the proliferation,migration,and invasion abilities of 4T1 cells treated with PFD at 2,4,6 and 8 mmol/L gradually weakened in a concentration-dependent manner.Among them,when the concentration of PFD was 8 mmol/L,it significantly inhibited the proliferation,migra-tion,and invasion abilities of 4T1 cells(P<0.001);Furthermore,molecular docking indicated that there were hydrogen bonds and hydrophobic interactions between PFD and NAT1.Conclusion:The concentration of PFD at 8 mmol/L reduces the prolifera-tion,migration,and invasion abilities of triple-negative breast cancer 4T1 cells by inhibiting the expression of NAT1 protein.关键词
三阴性乳腺癌/吡非尼酮/增殖/迁移/侵袭Key words
Triple negative breast cancer/Pirfenidone/Proliferation/Migration/Invasion分类
医药卫生引用本文复制引用
亢世景,王娴娴,余庆,史恩赐,徐丽,刘海琴,叶思思,周静..吡非尼酮对三阴性乳腺癌4T1细胞增殖、迁移和侵袭影响的机制研究[J].海南医科大学学报,2026,32(10):721-729,9.基金项目
This study was supported by the Guizhou Science and Technology Plan Project[Guizhou Science and Technology Cooperation Foundation-ZK(2024)General 108] （2024）
Guizhou Provincial Health Commission's Science and Technology Grant Program(gzwkj2024-095) （gzwkj2024-095）
Student Innovation and Entrepreneurship Project of Guizhou Medical University(20222123009) 贵州省科技计划项目[黔科合基础-ZK(2024)一般108] （20222123009）
贵州省卫生健康委科学技术基金(gzwkj2024-095) （gzwkj2024-095）
贵州医科大学大学生创新创业项目(20222123009) （20222123009）
