海南医科大学学报2026，Vol.32Issue(10)：751-758,8.DOI:10.13210/j.cnki.jhmu.20250408.004
雪上一枝蒿总生物碱及其三种生物碱类单体对MH7A细胞毒性及抗炎活性研究
Cytotoxic and anti-inflammatory activities of Aconitum brachypodum Diels total alkaloids and its three alkaloid monomers on MH7A cells
摘要
Abstract
Objective:To investigate the toxicity and in vitro anti-inflammatory activity of Aconitum brachypodum Diels total alkaloids and its three alkaloid monomers of on MH7A cells.Methods:MH7A cells were selected as experimental subjects,and the effects of total alkaloids of Aconitum brachypodum Diels alkaloids and its three alkaloid monomers on the cell viability of MH7A were determined by CCK-8 assay;The effect of total alkaloids of Aconitum brachypodum Diels alkaloids and its three al-kaloid monomers on the migration of MH7A cells was examined by the cell-scratch method;An in vitro rheumatoid arthritis(RA)cell model was established by tumor necrosis factor(TNF-α)stimulation of MH7A cells,and the expression of the inflam-matory factors prostaglandin E2(PGE-2),interleukin-6(IL-6),and interleukin-1β(IL-1β)in the supernatants of the cells was determined by ELISA.Results:The maximum cytotoxicity-free drug concentrations of each drug according to the CCK-8 assay were 1 140 μg/mL for Bullatine A,750 μg/mL for Bullatine B,100 μg/mL for total alkaloids of Aconitum brachypodum Diels,and 50 μg/mL for Aconitine officinale;The order of the inhibitory effects on the migration of MH7A cells in each dosing group was as follows:Aconitine>Bullatine B>total alkaloids of Aconitum brachypodum Diels>Bullatine A;Total alkaloids of Aco-nitum brachypodum Diels,Bullatine A,Bullatine B,and Aconitine inhibited the amount of PGE2,IL-6,and IL-1β production in MH7A cells after TNF-α induction.Conclusion:Total alkaloids of Aconitum brachypodum Diels,Bullatine A,Bullatine B,and Aconitine all inhibited the secretion of cytokines PGE2,IL-6,and IL-1β from MH7A cells in a concentration-dependent manner.关键词
雪上一枝蒿/总生物碱/生物碱类单体/MH7A细胞/抗炎活性Key words
Aconitum brachypodum Diels/Total alkaloids/Alkaloid monomers/MH7A cells/Anti-inflammatory activity分类
医药卫生引用本文复制引用
陈磊,陈晓兰,邓铋莉,谌文元,邓琴,范耕齐..雪上一枝蒿总生物碱及其三种生物碱类单体对MH7A细胞毒性及抗炎活性研究[J].海南医科大学学报,2026,32(10):751-758,8.基金项目
This study was supported by the Guizhou Province Basic Research Program(Natural Science Category)[Guizhou Science and Technology Cooperation Foundation-Basic-ZK(2023)General 407] （Natural Science Category）
Enhancement of the Capacity of the National Miao Medicine Engineering Research Center[Guizhou Science and Technology Cooperation Foundation-Medium and Long-Term Introduction and Development(2023)006] （2023）
Guizhou Province Project for High-Level Innovative Talents[Guizhou Science and Technology Cooperation Foundation-Platform Talent-GCC(2023)037] 贵州省基础研究计划(自然科学类)[黔科合基础-ZK(2023)一般 407] （2023）
国家苗药工程技术研究中心能力提升[黔科合中引地(2023)006] （2023）
贵州省高层次创新型人才项目[黔科合平台人才-GCC(2023)037] （2023）
