中医学报2026，Vol.41Issue(6)：1309-1314,6.DOI:10.16368/j.issn.1674-8999.2026.06.195
基于P2X2、M3和β3-AR受体探讨五苓散改善骶髓损伤后神经源性膀胱尿潴留的作用机制
Mechanism of Wuling Powder in Improving Neurogenic Bladder Urinary Retention in Rats After Sacral Spinal Cord Injury Based on P2X2,M3,and β3-AR Receptors
摘要
Abstract
Objective:To investigate the effect of Wuling Powder on the improvement of urinary retention in a rat model of neurogenic bladder(NB)after sacral spinal cord injury and its potential mechanism.Methods:A bladders with detrusor areflexia NB rat model was established by Hassan Shaker spinal cord transection.Rats were divided into a sham operation group,model group,and low,medium,and high dose Wuling Powder groups(1.5,3,6 g·kg-1),with 10 rats in each group.After 20 consecutive days of intragastric adminis-tration,HE staining was used to observe the pathological morphology of rat bladder tissue.qPCR and Western blot were used to detect the mRNA and protein expression levels of purinergic receptor subtype 2(P2X2),β-adrenergic receptor subtype 3(β3-AR),and muscarinic receptor subtype 3(M3-R)in rat bladder tissue.ELISA was used to detect serum dopamine(DA)levels,and a colorimet-ric method was used to detect adenosine triphosphate(ATP)levels in serum.Results:Compared with the sham surgery group,the BBB scores of rats in the model group were significantly decreased(P<0.01);hematoxylin and eosin(HE)staining of bladder tissue showed loose arrangement of connective tissue with inflammatory cell infiltration in the model group;bladder tissue β3-AR mRNA and protein expression levels were significantly increased,M3-R mRNA and protein expression levels were significantly decreased,and P2X2 protein expression levels were significantly increased(P<0.01);serum DA and ATP content were both significantly decreased(P<0.05).Compared with the model group,the BBB scores of rats in the middle-and high-dose groups of Wuling Powder were significantly increased(P<0.05),and the pathological abnormalities in the bladder tissue were alleviated;bladder tissue β3-AR mRNA and protein expression levels were significantly decreased,M3-R mRNA and protein expression levels were significantly in-creased,and P2X2 protein expression levels were significantly decreased(P<0.05);serum DA and ATP content were both significantly increased(P<0.05).Conclusion:Wuling Powder may improve urinary retention symptoms in rats after spinal cord injury by regulating the expression of P2X2,M3-R,and β3-AR,promoting the recovery of neural function and energy metabolism.关键词
五苓散/神经源性膀胱/尿潴留/P2X2/M3-R/β3-AR/骶髓损伤Key words
Wuling Powder/neurogenic bladder/urinary retention/P2X2/M3-R/β3-AR/sacral spinal cord injury分类
医药卫生引用本文复制引用
赵树克,肖小乔,赵照禾,林圳,王杨,张琳,王振亮..基于P2X2、M3和β3-AR受体探讨五苓散改善骶髓损伤后神经源性膀胱尿潴留的作用机制[J].中医学报,2026,41(6):1309-1314,6.基金项目
河南省重点研发专项项目(231111310300) （231111310300）
河南省郑州市协同创新专项项目(2023XTCX049) （2023XTCX049）
