黑龙江农业科学Issue(5)：1-8,8.DOI:10.11942/j.issn1002-2767.2026.05.0001
连作与轮作对大豆和玉米根际土壤细菌微生态的影响
Influences of Continuous Cropping and Rotation on Rhizosphere Soil Bacterial Microecology of Soybean and Maize
摘要
Abstract
In order to explore the influence of continuous cropping and rotation cropping systems on the diversity of the rhizosphere soil microbial community of soybean and maize,and to provide a reference basis for the optimization of crop soil and the improvement of crop product quality.The traditional microbial cultivation technology was combined with the Illumina MiSeq high-throughput sequencing technology.The research was carried out from the aspects of the composition of the community structure,the changes in abundance,and the correlation between the distribution of the bacterial community and soil environmental factors of the rhizosphere soil bacteria of crops under different cropping systems.Under the long-term continuous cropping of soybean,continuous cropping of maize,and soybean-maize rotation planting patterns,the diversity of the bacterial community was in the order of continuous cropping of soybean＞continuous cropping of maize＞soybean-maize rotation;the richness of the bacterial community showed continuous cropping of soybean＞continuous cropping of maize＞soybean-maize rotation.The structures of the bacterial communities in the soils of continuous cropping of soybean,continuous cropping of maize,and soybean-maize rotation at the phylum level were basically the same.The dominant bacterial groups were mainly Acidobacteriota(17.25％-32.14％),Proteobacteria(19.25％-24.42％),and Bacteroidota(9.58％-18.7 3％).However,the relative abundances of Proteobacteria(24.40％)and Bacteroidota(18.7 6％)in the soybean-maize rotation were significantly higher than those in the continuous cropping of soybean(19.41％)and continuous cropping of maize(19.32％),while the relative abundance of Acidobacteriota(17.27％)was significantly lower than that in the continuous cropping of soybean(29.09％)and continuous cropping of maize(32.07％).The content of available potassium(AK)in the soil was the main environmental factor affecting the community distribution of the dominant bacterial groups Acidobacteriota,Proteobacteria,and Bacteroidota.Continuous cropping of soybean can increase the diversity and richness of bacteria,and the community structure of microorganisms can be changed by regulating the content of available potassium(AK).关键词
高通量测序/种植制度/群落结构/丰度/环境因子Key words
high-throughput sequencing/planting system/community structure/abundance/environmental factor引用本文复制引用
齐国超,王丽娜,姜泽宇,顾鑫,王迪..连作与轮作对大豆和玉米根际土壤细菌微生态的影响[J].黑龙江农业科学,2026,(5):1-8,8.基金项目
黑龙江省省属科研院所科研业务费项目(CZKYF2024-1-C005) （CZKYF2024-1-C005）
黑龙江省农业科技创新跨越工程农业关键技术重点攻关项目(CX23GG09). （CX23GG09）
