黑龙江农业科学Issue(5)：25-32,8.DOI:10.11942/j.issn1002-2767.2026.05.0025
苦楝白化叶SGR基因克隆与生物信息学分析
Cloning and Bioinformatics Analysis of SGR Gene from Albino Leaves of Melia azedarach
摘要
Abstract
In order to investigate the causes of leaf albinism in Melia azedarach,this study used green leaves and albino leaves as research subjects.Through freehand sectioning and microscopic observation of cell color distribution,the contents of chlorophyll and carotenoids were measured.Additionally,the key chlorophyll degradation gene SGR was cloned and subjected to bioinformatics analysis.The results showed that in the albino leaves,green cells were almost undetectable in both the palisade and spongy tissues.The total chlorophyll content in green leaves was 1.66 mg·g-1,significantly higher than that in albino leaves(1.02 mg·g-1).Similarly,the carotenoid content in green leaves was 0.34 mg·g-1,markedly higher than that in albino leaves.Cloning and analysis of the SGR gene in M.azedarach revealed that the full-length CDS of this gene was 345 bp,encoding 115 amino acids.The SGR protein was alkaline,hydrophobic,non-secretory,and non-transmembrane,with its secondary structure predominantly composed of random coils.Subcellular localization prediction indicated its presence in the cytoplasm.Phylogenetic analysis showed that the SGR gene of M.azedarach was most closely related to that of Sarracenia purpurea.Therefore,the reduction in chlorophyll is the key factor leading to leaf albinism in M.azedarach.Further in-depth research on the SGR gene,a critical gene involved in chlorophyll degradation,will contribute to elucidating the molecular mechanisms underlying the albino leaf phenotype in M.azedarach.关键词
苦楝/SGR/白化叶/生物信息学分析Key words
Melia azedarach L./SGR/albino leaf/bioinformatics analysis引用本文复制引用
阿斯拉姆·法尔汉,郑春磊,穆家浩,杜童,吴欣欣..苦楝白化叶SGR基因克隆与生物信息学分析[J].黑龙江农业科学,2026,(5):25-32,8.基金项目
国家自然科学基金(32001358) （32001358）
淮阴工学院博士科研启动项目(Z301B20107). （Z301B20107）
