河南农业科学2026，Vol.55Issue(5)：63-72,10.DOI:10.15933/j.cnki.1004-3268.2026.05.007
人参EIL基因家族的鉴定及其在重金属胁迫下的表达分析
Identification of the EIL Gene Family in Panax ginseng and Its Expression Analysis under Heavy Metal Stress
摘要
Abstract
EIL(Ethylene-insensitive3)transcription factors were core regulatory factors in plant ethylene signal transduction and stress response,involved in various physiological and biochemical processes in plants.To elucidate the distribution and function of the EIL family in Panax ginseng,PgEIL gene family members were screened based on the Panax ginseng genome,and their protein properties,phylogenetic relationships,gene structures,tissue expression patterns,etc.were analyzed through bioinformatics.The results showed that a total of 11 PgEIL genes were screened,and the physicochemical property prediction analysis showed that they were acidic hydrophilic unstable nucleoproteins.PgEIL genes were unevenly distributed across 10 chromosomes and exhibited 6 pairs of linear relationships.The PgEIL genes had a highly conserved EIN3 domain,and all contained Motif2,Motif5,and Motif9 conserved motifs.Phylogenetic analysis showed that the PgEIL genes could be divided into three different evolutionary branches,and members of the same branch exhibited similar characteristics,such as PgEIL3 and PgEIL4.The promoter regions of PgEIL genes were rich in hormone,stress,and light response elements,and all contained light response elements.The transcriptome data analysis results showed that the PgEIL genes had typical tissue expression specificity,with low expression levels in roots and high expression levels in fruits,suggesting that members of the PgEIL family were involved in the growth and development of Panax ginseng.The expression of PgEIL genes under heavy metal stress was analyzed by qRT-PCR.The results showed that Pb,Cd,Cu,and Zn could induce the expression of PgEIL11 gene.PgEIL3,PgEIL5,PgEIL7,and PgEIL8 genes were highly sensitive to Pb and significantly upregulated in expression levels.PgEIL9 gene was activated under Cd treatment but inhibited under Cu and Zn stress,and heavy metal stress could induce the expression of most PgEIL genes.In summary,the PgEIL genes play an important regulatory role in Panax ginseng's response to heavy metal stress.关键词
人参/EIL转录因子/重金属胁迫/表达分析/生物信息学Key words
Panax ginseng/EIL transcription factor/Heavy metal stress/Expression analysis/Bioinformatics分类
农业科技引用本文复制引用
梁勇,吴磊..人参EIL基因家族的鉴定及其在重金属胁迫下的表达分析[J].河南农业科学,2026,55(5):63-72,10.基金项目
国家自然科学基金项目(82003970) （82003970）
