解放军医学杂志2026，Vol.51Issue(5)：667-674,8.DOI:10.11855/j.issn.0577-7402.0003.2025.1013
BIK在胰腺癌中的表达特征及临床预后价值
Expression of BIK in pancreatic cancer and its clinical prognostic value
摘要
Abstract
Objective To explore the expression pattern of Bcl-2 interacting killer protein(BIK)in pancreatic cancer,its relationship with tumor microenvironment and patient prognosis,and to evaluate its potential as a prognostic biomarker and therapeutic target.Methods(1)Expression profile data of pancreatic cancer were obtained from The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)datasets.Differentially expressed genes were screened using bioinformatics methods,and BIK was identified as the research target based on survival analysis.Furthermore,Gene Ontology(GO)enrichment analysis and the Gene Expression Profiling Interactive Analysis(GEPIA)database were used to evaluate its potential biological function and its correlation with collagen deposition and immune cell infiltration.(2)Tissue samples from 56 pancreatic cancer patients admitted to Lanzhou University Second Hospital between December 2020 and September 2023 were retrospectively collected.Hematoxylin-eosin(HE),immunohistochemistry(IHC),and Sirius red staining were performed to assess the correlation between BIK expression and patients'clinicopathological features as well as prognosis.Results BIK was significantly overexpressed in pancreatic cancer tissues and associated with poor prognosis(P<0.05).It was enriched in extracellular matrix regions containing collagen and positively correlated with the expression of collagen type Ⅳ alpha 1 chain(COL4A1)and lysyl oxidase(LOX)(r=0.39,0.23,P<0.001).BIK expression was significantly inversely correlated with M1 macrophage infiltration(r=-0.166,P<0.001),but showed a significant positive correlation with the expression of immunosuppressive markers TGFB1 and TGFB2(r=0.53,0.26,P<0.001).High BIK expression was an independent risk factor affecting the overall survival(OS)in pancreatic cancer patients(P=0.0014),and it was closely correlated with the proportion of collagen regions(R²=0.5375,P<0.001)and the number of CD86-positive cells(R²=0.4345,P<0.001).Receiver operating characteristic(ROC)curve analysis demonstrated that the expression of BIK protein had certain predictive value for the survival prognosis of pancreatic cancer patients(AUC=0.7288,P=0.0034).In addition,BIK expression level was significantly correlated with lymph node metastasis(N stage)(P=0.001).Conclusions BIK is upregulated in pancreatic cancer and may promote tumor progression by promoting collagen deposition and inhibiting M1 macrophage infiltration,it is expected to be a promising prognostic biomarker and therapeutic target.关键词
胰腺癌/Bcl-2相互作用杀伤蛋白/预后/细胞外基质/免疫浸润Key words
pancreatic cancer/Bcl-2-interacting killer protein/prognosis/extracellular matrix/immune infiltration分类
医药卫生引用本文复制引用
庆会国,杨丹锋,侯岩松,王田,焦作义,俞泽元..BIK在胰腺癌中的表达特征及临床预后价值[J].解放军医学杂志,2026,51(5):667-674,8.基金项目
This work was supported by the National Natural Science Foundation of China(82160487),the Lanzhou Youth Science and Technology Talent Innovation Project(2023-QN-15),and the Lanzhou University Undergraduate Innovation and Entrepreneurship Training Program Project(20250020079)国家自然科学基金(82160487) （82160487）
兰州市青年科技人才创新项目(2023-QN-15) （2023-QN-15）
兰州大学大学生创新创业训练计划项目(20250020079) （20250020079）
