激光生物学报2026，Vol.35Issue(2)：97-110,120,15.DOI:10.3969/j.issn.1007-7146.2026.02.001
激光生物调控技术:从非特异性到特异性调控
Laser Biomodulation:from Nonspecific to Specific Modulation
摘要
Abstract
As a non-invasive method,laser biological modulation technology can manipulate specific molecular processes and functions of cells or organisms at both in vitro and in vivo levels through different mechanisms.This review analyzes the light absorption spectrum of distinct intracellular endogenous photosensitive molecules,elaborates on the main physical mechanisms of laser-tissue interaction,including the photothermal effect and photochemical effect by linear absorption,as well as the multiphoton excitation and weak ionization effect driven by high photon density.This review discusses the possibility of direct specific interactions between laser and different molecules in cells.Based on this,the regulatory mechanisms,applications,technical characteristics and limitations of low-level laser therapy and femtosecond-laser specific modulation technology are derived.This paper further analyzes the biological modulation strategies,technical advantages and limitations of optogenetics and optical uncaging technology,and conducts a detailed comparison of these four technologies.Finally,aiming at the current challenges such as penetration depth limitations caused by tissue scattering,lack of molecular specificity and poor biocompatibility,the causes and potential solutions are specifically elaborated.关键词
激光调控/光生物调节作用/光遗传学/光解笼锁/飞秒激光Key words
laser modulation/photobiomodulation/optogenetics/photo-uncaging/femtosecond laser分类
生物科学引用本文复制引用
于桥,贺号..激光生物调控技术:从非特异性到特异性调控[J].激光生物学报,2026,35(2):97-110,120,15.基金项目
国家重点研发计划战略性科技创新合作专项(2025YFE0203084). （2025YFE0203084）
