中国当代医药2026，Vol.33Issue(11)：4-10,7.DOI:10.3969/j.issn.1674-4721.2026.11.01
安罗替尼通过PI3K/Akt信号通路调控人肝癌细胞株HepG2增殖迁移的机制
Mechanism of Anlotinib in regulating the proliferation and migration of human hepatocellular carcinoma cell line HepG2 through the PI3K/Akt signaling pathway
摘要
Abstract
Objective To investigate the impact of Anlotinib on the proliferation and migration of human hepatocellular car-cinoma cells and to determine whether its mechanism involves the PI3K/Akt signaling pathway.Methods Anlotinib and the PI3K inhibitor LY294002 were applied to passaged human hepatocellular carcinoma cell line HepG2.Experimental groups included Anlotinib alone,the PI3K inhibitor LY294002 alone,a combination of both drugs,and a control group.Cell proliferation,clonogenic formation,and migration capabilities were assessed using the CCK-8 assay and the cell scratch wound healing assay,respectively.Additionally,Western blotting was employed to examine the effects of Anlotinib and LY294002 on the expression of matrix metalloproteinase-3(MMP-3)and phosphorylated Akt(p-Akt)proteins in HepG2 cells.Results After 24 hours of treatment with Anlotinib,the optimal concentration for HepG2 cells was determined to be 5 000 nmol/L,achieving a cell inhibition rate of 49.649％.Compared with the control group,the proliferation rates of HepG2 cells in both the 5 000 nmol/L Anlotinib treatment group and the 8 μmol/L LY294002 treatment group were significantly lower,the differences were statistically significant(P＜0.05).In the clonogenic formation and migration as-says,the clonogenic formation rates and migration rates of the Anlotinib group,the LY294002 group,and the combination group were all lower than those of the control group,with statistically significant differences(P＜0.05).Furthermore,the clonogenic formation rates and migration rates in the combination group were lower than those in either the Anlotinib alone group or the LY294002 alone group,the differences were statistically significant(P＜0.05).Regarding protein expression,the expression levels of p-Akt and MMP-3 proteins in the anlotinib group,LY294002 group,and combination group were lower than those in the control group,the differences were statistically significant(P＜0.05).Additionally,the expression levels of both p-Akt and MMP-3 proteins in the combination group were significantly lower than those in either the Anlotinib alone group or the LY294002 alone group,the differences were statistically significant(P＜0.05).Conclusion Anlotinib exhibits inhibitory effects on the proliferation and migration of hepatocellular carcinoma HepG2 cells.The underlying mechanism of this inhibitory effect may be associated with the suppression of the PI3K/Akt signaling pathway.关键词
安罗替尼/LY294002/蛋白质金属基质-3/细胞增殖/HepG2Key words
Anlotinib/LY294002/Matrix metalloproteinase-3/Cell proliferation/HepG2分类
医药卫生引用本文复制引用
张静宇,张鸿雁,赵晶,杨洁,齐云飞,孟祥海..安罗替尼通过PI3K/Akt信号通路调控人肝癌细胞株HepG2增殖迁移的机制[J].中国当代医药,2026,33(11):4-10,7.基金项目
山东省医药卫生科技发展计划项目(202103101086). （202103101086）
