集美大学学报（自然科学版）2026，Vol.31Issue(3)：253-263,11.DOI:10.19715/j.jmuzr.2026.03.01
拟穴青蟹钙调蛋白基因的分子特性及蜕皮作用分析
Molecular Characterizations of Calmodulin Gene and Analysis of Molting Process in Scylla paramamosain
摘要
Abstract
In this study,the CaM gene was cloned from the mud crab Scylla paramamosain.The full-length cDNA of SpCaM was 1980 bp,and its 450 bp open reading frame(ORF)was isolated and characterized.Clust-alW and phylogenetic analysis showed that SpCaM was closely related to the CaM proteins from other crustace-ans.Quantitative real-time PCR revealed that during molt cycle of early juvenile S.paramamosain,the SpCaM mRNA levels reached the peak at the intermolt stage,and its expression pattern was similar to that of SpMIH.Further RNA interference experiment showed that injection of double stranded SpMIH(ds SpMIH)into the juven-ile crabs resulted in significant reduction of SpMIH and SpCaM transcripts at 48 h post injection.These findings indicated that SpCaM participates in regulating molting of S.paramamosain and was likely regulated by SpMIH.关键词
拟穴青蟹/钙调蛋白/蜕皮/蜕皮抑制激素Key words
Scylla paramamosain/calmodulin/molt/molt inhibiting hormone分类
农业科技引用本文复制引用
贾佩潭,王小鹏,叶海辉..拟穴青蟹钙调蛋白基因的分子特性及蜕皮作用分析[J].集美大学学报（自然科学版）,2026,31(3):253-263,11.基金项目
国家自然科学基金项目(42206132) （42206132）
福建省自然科学基金项目(ZQ2022022) （ZQ2022022）
