江苏大学学报（医学版）2026，Vol.36Issue(3)：192-196,5.DOI:10.13312/j.issn.1671-7783.y250035
短期高糖饮食对哮喘小鼠气道炎症的影响
Effects of short-term high-sugar diet on airway inflammation in asthmatic mice
摘要
Abstract
Objective:To investigate the role and mechanism of short-term high-sugar diet in airway inflammation in asthmatic mice by regulating the proportion of Th cells.Methods:BALB/c mice were randomly divided into control group,high-sugar diet group,asthma group and high-sugar asthma group.Mice in control group and asthma group were fed with normal drinking water,while those in high-sugar diet group and high-sugar asthma group were fed with 20%glucose water.Ovalbumin was used to establish asthmatic model in asthma group and high-sugar asthma group.HE and PAS staining were used to observe lung histopathology and goblet cell hyperplasia.Wright's staining was used to detect the percentage of eosinophils.Flow cytometry was used to analyze the proportion of Th cells,and qRT-PCR was used to detect the expression of cytokines.Results:Compared with the control group,airway inflammation in the high-sugar diet group showed no obvious changes,and there was no significant difference in Th cells proportion and cytokine expression(P>0.05).However,when compared to the control group,airway inflammation in both the asthma group and high-sugar asthma group was obvious,the proportions of eosinophils,Th2 and Th17 cells were significantly increased,the proportions of Th1 cells and Treg cells were significantly decreased,the expressions of IL-4 and IL-17 mRNA were significantly up-regulated,and the expression of TGF-β mRNA was significantly down-regulated(all P<0.05).The expression of IFN-γ mRNA in the high-sugar asthma group was significantly lower than that in the control group(P<0.05).Compared with the asthma group,the proportions of Th1 and Treg cells in the high-sugar asthma group were significantly decreased,the proportions of Th2 and Th17 cells were significantly increased,the expressions of IL-4 and IL-17 mRNA were significantly up-regulated,and the expressions of TGF-β mRNA was significantly down-regulated(all P<0.05).Conclusion:Short-term high-sugar diet can up-regulate the proportions of Th2 and Th17 cells,down-regulate the proportions of Th1 and regulatory T cells,and subsequently aggravate airway inflammation in asthmatic mice.关键词
高糖饮食/辅助性T细胞/细胞因子/哮喘/气道炎症/免疫调节Key words
high sugar diet/T helper cell/cytokines/asthma/airway inflammation/immunomodulation分类
医药卫生引用本文复制引用
叶济贤,王诏玉,汪雪峰..短期高糖饮食对哮喘小鼠气道炎症的影响[J].江苏大学学报（医学版）,2026,36(3):192-196,5.基金项目
国家自然科学基金资助项目(81871243) （81871243）
江苏省社会发展项目(BE2017697) （BE2017697）
江苏省"六大人才高峰"项目(WSN-009) （WSN-009）
镇江市中医妇科病临床医学研究中心开放课题(SS202204-KFB02) （SS202204-KFB02）
