现代畜牧兽医Issue(4)：24-28,5.DOI:10.20154/j.cnki.issn1672-9692.2026.04.005
警犬性控精液X、Y精子比例检测方法的建立
Establishment of a method to detect the ratio of X and Y sperm for sex-sorted semen in police dogs
摘要
Abstract
To establish an economical,rapid,and accurate method for detecting the proportion of X-and Y-chromosome-bearing sperm in police dog semen,specific primers were designed targeting canine AKAP4(an X-sperm marker)and SRY gene(a Y-sperm marker).A series of plasmid standards with known copy numbers were constructed.Standard curves for the AKAP4 and SRY genes were established by real-time quantitative PCR.The method was validated by detecting mixed plasmid samples,commercially available sex-sorted semen,and unsorted semen.The results showed that the standard curve for the AKAP4 gene was y=-3.226x+40.412,with an determination coefficient(R²)of 0.993 and an efficiency of 104%.The standard curve for the SRY gene was y=-3.17x+37.225,with an R² of 0.998 and an efficiency of 111%,indicating good linearity.At different dilution ratios and mixing proportions,there were no significant differences between the detected Y-sperm values and the theoretical values(P>0.05),and the coefficients of variation(CV)between groups ranged from 1.48%to 2.73%,demonstrating that the method is stable and reliable.This study successfully established a simple and accurate method for detecting the X/Y sperm ratio in police dog semen,which can be used for quality assessment of sex-sorted semen.关键词
警犬/性控精液/精子比例/荧光定量PCRKey words
Police dog/Sex-sorted semen/Sperm ratio/Real-time fluorescence quantitative PCR分类
农业科技引用本文复制引用
潘彩霞..警犬性控精液X、Y精子比例检测方法的建立[J].现代畜牧兽医,2026,(4):24-28,5.基金项目
2025年度辽宁省教育厅高校基本科研项目(LJ212510175016) （LJ212510175016）
