现代畜牧兽医Issue(5)：58-64,7.DOI:10.20154/j.cnki.issn1672-9692.2026.05.012
新疆石河子地区规模化鸡场空肠弯曲杆菌分离鉴定及生物学特性分析
Isolation,identification,and biological characteristics analysis of Campylobacter jejuni from large-scale chicken farms in Shihezi Area,Xinjiang
摘要
Abstract
This study aimed to investigate the prevalence and biological characteristics of Campylobacter jejuni in large-scale chicken farms in the Shihezi Area of Xinjiang.A total of 198 anal swab samples were collected from four local large-scale farms.Isolates were identified through morphological and biochemical characterization,as well as multiplex PCR.Antimicrobial susceptibility testing was performed using the K-B disc diffusion method.Drug resistance genes and virulence genes were detected via PCR.Biofilm formation ability was assessed using the crystal violet staining method,and a mouse pathogenicity test was conducted.The results showed that eight strains of Campylobacter jejuni were isolated from the 198 anal swab samples,with an isolation rate of 4.04％.Three of the isolates exhibited strong biofilm-forming ability.All eight isolates were multidrug-resistant,showing 100.0％resistance to trimethoprim-sulfamethoxazole,while no resistance was observed to cefotaxime,enrofloxacin,azithromycin,erythromycin,or streptomycin.Regarding drug resistance genes,the highest detection rate was for L22(75.0％,6/8),while the lowest was for aph(2'')-If(25.0％,2/8).Virulence gene analysis revealed that wlaN and pldA had the highest detection rates(62.5％,5/8).All mice infected with the isolates died within 1 to 3 days,and significant pathological changes were observed in the heart,liver,spleen,lungs,and kidneys.The study indicates that Campylobacter jejuni in large-scale chicken farms in the Shihezi Area of Xinjiang exhibits high levels of drug resistance and pathogenicity.These findings provide a reference for the prevention and control of Campylobacter jejuni.关键词
空肠弯曲杆菌/鸡/分离鉴定/耐药特性/耐药基因/毒力基因Key words
Campylobacter jejuni/Chicken/Isolation and identification/Antimicrobial resistance characteristics/Resistance genes/Virulence genes分类
农业科技引用本文复制引用
柳汀育,吴洁,刘良波,赵天艺,张辉,曾文轩,窦立静..新疆石河子地区规模化鸡场空肠弯曲杆菌分离鉴定及生物学特性分析[J].现代畜牧兽医,2026,(5):58-64,7.基金项目
国家自然科学基金(31860641) （31860641）
兵团重点领域科技攻关(2023AB007-01) （2023AB007-01）
