南京中医药大学学报2026，Vol.42Issue(5)：677-688,12.DOI:10.14148/j.issn.1672-0482.2026.0677
杏贝止咳颗粒治疗急性支气管炎的功效物质及作用机制研究
Study on the Effective Substances and Mechanism of Action of Xingbei Zhike Granules in the Treatment of Acute Bronchitis
摘要
Abstract
OBJECTIVE To elucidate the effective substances and mechanism of action of Xingbei Zhike Granules in treating acute bronchitis.METHODS The potential effective substances and action mechanisms of Xingbei Zhike Granules were predicted through network pharmacology.Subsequently,the effective substances were enriched and purified by macroporous adsorption resin and separated.The anti-inflammatory effective components in the effective substances were screened through an in vitro inflammatory cell model,and a group of effective components(ECPs)was established.Finally,a mouse model of acute bronchitis was constructed by in-tranasal instillation of LPS to evaluate the pharmacological effects of Xingbei Zhike Granules,effective substances,and ECPs.RT-qPCR was used to detect the expression of main mRNAs and Western blot was used to detect the proteins of inflammatory mediators and the NLRP3 pathway,and the diversity of intestinal flora in mouse feces was analyzed through microbiota analysis.RESULTS Network pharmacology predicted that the flavonoid components of Xingbei Zhike Granules had anti-inflammatory effects and might treat acute bronchitis through the NLRP3 signaling pathway.The 60%ethanol elution fraction obtained by macroporous adsorption resin was the total flavonoid fraction(XBZK-60).Five flavonoid compounds were isolated from the total flavonoid fraction,namely liquiritin,oroxin A,chrysin-7-O-β-D-glucuronide,chrysin-7-O-gentiobioside,and oroxin B.Oroxin A,chrysin-7-O-β-D-glucuronide and oroxin B were screened as ECPs through an in vitro inflammatory cell model.The in vivo pharmacodynamic results showed that compared with the model group,the Xingbei Zhike Granules group,XBZK-60 group,and ECPs group could significantly reduce the content of IL-6 and IL-1β in the bronchoalveolar lavage fluid of mice with acute bronchitis(P<0.05,P<0.01).RT-qPCR results indicated that each administration group could significantly reduce the mRNA expression of IL-6,IL-1α,IL-1β and TNF-α in the lung tissues of mice with acute bronchitis(P<0.01).Western blot results showed that each administration group could significantly down-regulate the protein expression of Cleaved Caspase-1,ASC,NLRP3,and TNF-α in the lung tissues of mice with acute bronchitis(P<0.05,P<0.01).The gut microbiota analysis showed that compared with the model group,the abundance and richness of gut micro-biota in each administration group were significantly increased,and the numbers of beneficial bacteria Lachnospira-ceae_NK4A136_group,Prevotellaceae_UCG-001,and Colidextribacter in the gut microbiota were significantly higher than those in the model group.CONCLUSION Total flavonoid components are the effective substances of Xingbei Zhike Granules in the treatment of acute bronchitis,and they can achieve anti-inflammatory effects by regulating the NLRP3 signaling pathway.Among them,oroxin A,oroxin B and chrysin-7-O-β-D-glucuronide are the key components of the total flavonoids.关键词
杏贝止咳颗粒/急性支气管炎/网络药理学/功效物质/肠道微生物/NLRP3信号通路/炎症/肺-肠轴Key words
Xingbei Zhike Granules/acute bronchitis/network pharmacology/effective substances/gut microbiota/NLRP3 signal-ing pathway/inflammation/lung-intestine axis分类
医药卫生引用本文复制引用
张智超,刘莉娜,李梦雅,花永娇,王守卓,张宏达,章晨峰,王振中,谢雪..杏贝止咳颗粒治疗急性支气管炎的功效物质及作用机制研究[J].南京中医药大学学报,2026,42(5):677-688,12.基金项目
国家工信部产业基础再造和制造业高质量发展专项(TC2308068) （TC2308068）
中药制药过程控制与智能制造技术国家重点实验室定向项目(SKL2023D01006) （SKL2023D01006）
