南京中医药大学学报2026，Vol.42Issue(5)：699-711,13.DOI:10.14148/j.issn.1672-0482.2026.0699
白花蛇舌草改善肝纤维化伴骨骼肌损伤机制研究
Study on the Mechanism of Hedyotis Diffusa in Improving Liver Fibrosis with Skeletal Muscle Injury
摘要
Abstract
OBJECTIVE To reveal the mechanism by which Hedyotis diffusa achieves synergistic therapeutic effects by regulating liver-muscle metabolic homeostasis through multiple targets.METHODS A mouse model of liver fibrosis with skeletal muscle injury was induced by CCl4;active components entering the blood were identified by HPLC-Q-TOF;111 key targets were screened by net-work pharmacology;their pathways of action were analyzed by KEGG enrichment;high affinity of rutin for tumor protein 53(TP53)and quercetin for tumor necrosis factor(TNF)was verified by molecular docking;and the effects of HDI on skeletal muscle mitochondrial function and respiratory chain gene expression were assessed by metabolomics.RESULTS HDI could simultaneously improve colla-gen deposition in liver tissue and skeletal muscle injury;KEGG enrichment analysis showed that it regulated metabolic disorders through the HIF-1 and Hippo signaling pathways;molecular docking verified the high affinity of rutin for TP53 and quercetin for TNF;metabolomics confirmed that HDI reversed mitochondrial dysfunction in skeletal muscle and repaired respiratory chain gene expres-sion.CONCLUSION This study breaks through the limitations of traditional single-target hepatic stellate cells therapy and provides a new strategy for liver-muscle comorbidity from the perspective of organ interaction.关键词
肝纤维化/白花蛇舌草/骨骼肌损伤/肌少症/肝-肌轴/代谢调控/异病同治Key words
liver fibrosis/Hedyotis diffusa/skeletal muscle injury/sarcopenia/liver-muscle axis/metabolic regulation/treatment of different diseases with the same method分类
医药卫生引用本文复制引用
张梦茹,刘欢,江若楠,熊舒华,张峰,吴红雁,孔德松,陈利..白花蛇舌草改善肝纤维化伴骨骼肌损伤机制研究[J].南京中医药大学学报,2026,42(5):699-711,13.基金项目
四大慢病国家科技重大专项(2024ZD0530800) （2024ZD0530800）
江苏省中医药科学技术发展计划项目(QN202304,ZD202402) （QN202304,ZD202402）
国家自然科学基金面上项目(82173874,82274339) （82173874,82274339）
江苏省高校优秀科技创新团队(2023) （2023）
江苏省心脑血管疾病与肿瘤防治工程技术研究中心(2022) （2022）
