南京中医药大学学报2026，Vol.42Issue(5)：722-733,12.DOI:10.14148/j.issn.1672-0482.2026.0722
基于UPLC-Q-Exactive Orbitrap-MS的射干麻黄汤入血成分研究
Identification of the Components of Shegan Mahuang Decoction Absorbed into Blood Based on UPLC-Q-Exactive Orbitrap-MS
摘要
Abstract
OBJECTIVE To identify and analyze the constituents in rat serum after oral administration of Shegan Mahuang De-coction,and to provide a reference for its further research on pharmacodynamics material basis.METHODS Male SD rats were used as experimental subjects and were administered the decoction of Shegan Mahuang Decoction at a dose of 18.9 g·kg-1 for 7 consecutive days.Two hours after the last administration,blood was collected from the abdominal aorta to obtain serum samples.Ultra-performance liquid chromatography-quadrupole-electrostatic field orbitrap mass spectrometry(UPLC-Q-Exactive Orbitrap-MS)was employed to analyze the serum of rats in both the blank control group and the administration group.Based on the retention time,accu-rate relative molecular weight,molecular fragment ion peaks provided by mass spectrometry,reference substance information,and rel-evant literature,the blood-entering components of Shegan Mahuang Decoction in the rats after gavage administration were identified.RESULTS A total of 30 components were identified,including 8 flavonoids,7 phenolic acids,3 alkaloids,4 glycosides,1 saponins,2 lig-nans,3 terpenoids and other compound components.CONCLUSION The thirty components absorbed into blood may be the potential bioactive components in Shegan Mahuang Decoction,providing a scientific basis for clarifying its material basis in pharmacodynamics.关键词
射干麻黄汤/UPLC-Q-Exactive Orbitrap-MS/入血成分/成分鉴定/裂解规律/射干/麻黄Key words
Shegan Mahuang Decoction/UPLC-Q-Exactive Orbitrap-MS/blood-entering components/component identification/fragmentation rule/Belamcandae rhizoma/Ephedrae herba分类
医药卫生引用本文复制引用
张溪,杨晓琳,张开源..基于UPLC-Q-Exactive Orbitrap-MS的射干麻黄汤入血成分研究[J].南京中医药大学学报,2026,42(5):722-733,12.基金项目
广州市科技局市校联合项目(2024A03J0039) （2024A03J0039）
2023年度广东省中医院中医药科学技术研究专项(YN2023MS22) （YN2023MS22）
