北方农业学报2026，Vol.54Issue(2)：36-47,12.DOI:10.12190/j.issn.2096-1197.2026.02.04
不同播种期西瓜糖分含量与蔗糖代谢酶活性动态变化
Dynamic changes in sugar content and sucrose-metabolizing enzyme activities of watermelon at different sowing times
摘要
Abstract
[Objective]To investigate the dynamic changes in sugar content and sucrose-metabolizing enzyme activities during watermelon fruit development at different sowing times,and to provide a reference for high-quality cultivation and quality improvement of watermelon.[Methods]The watermelon variety Qianli No.4 was used as the experimental material.A spring experiment(sowing time Ⅰ and sowing time Ⅱ)and autumn experiment(sowing time Ⅲ and sowing time Ⅳ)were set.Fruit sugar content and sucrose-metabolizing enzyme activities were measured at different days after pollination.[Results]Glucose content was highest at 20 d after pollination in sowing time Ⅰ,26 d after pollination in sowing time Ⅱ,35 d after pollination in sowing time Ⅲ,and 26 d after pollination in sowing time Ⅳ(39.67,46.94,33.12,and 29.01 mg/g,respectively).Fructose content was highest at 23 d after pollination in sowing time Ⅰ,29 d after pollination in sowing time Ⅱ,44 d after pollination in sowing time Ⅲ,and 44 d after pollination in sowing time Ⅳ(76.37,66.33,65.83,and 70.00 mg/g,respectively).Sucrose content was highest at 29 d after pollination in sowing time Ⅰ,29 d after pollination in sowing time Ⅱ,44 d after pollination in sowing time Ⅲ,and 41 d after pollination in sowing time Ⅳ(64.57,43.61,38.63,and 38.54 mg/g,respectively).Total sugar content was highest at 29 d after pollination in sowing time Ⅰ,29 d after pollination in sowing time Ⅱ,44 d after pollination in sowing time Ⅲ,and 41 d after pollination in sowing time Ⅳ(153.20,141.93,118.25,and 119.15 mg/g,respectively).The activity of neutral invertase(NI)was highest at 26 d after pollination in sowing time Ⅰ,14 d after pollination in sowing time Ⅱ,44 d after pollination in sowing time Ⅲ,and 23 d after pollination in sowing time Ⅳ[1 757.33,1 837.93,1 607.89,and 1 890.57 μg/(min·g),respectively].The activity of acid invertase(AI)was highest at 23 d after pollination in sowing time Ⅰ,7 d after pollination in sowing time Ⅱ,38 d after pollination in sowing time Ⅲ,and 23 d after pollination in sowing time Ⅳ[1 645.44,1 541.99,1 404.88,and 1 604.70 μg/(min·g),respectively].In both spring and autumn fruits,the contents of sucrose,glucose,fructose,and total sugar were significantly or extremely significantly correlated with the activity of sucrose phosphate synthase(SPS),sucrose synthase[(synthetic direction),SSs],sucrose synthase[(cleavage direction),SSc)],and the net activity of sucrose-metabolizing enzymes(P＜0.05 or P＜0.01).[Conclusion]For watermelon fruits at different sowing times,fructose content is higher during the development stages,glucose content is higher at the early development stages,sucrose content is higher at the late development stages,and total sugar and glucose contents are higher in spring than in autumn;the activities of sucrose-metabolizing enzymes show fluctuating changes,and all invertase activities were higher.The differences in fruit sugar accumulation are related to the activities of sucrose-metabolizing enzymes.关键词
西瓜/播种期/糖分含量/蔗糖代谢酶/相关性分析Key words
Watermelon/Sowing time/Sugar content/Sucrose-metabolizing enzyme/Correlation analysis分类
农业科技引用本文复制引用
沈虹,孟佳丽,吴绍军,杨年福,张黎杰,周玲玲,田福发,刘书华,余翔..不同播种期西瓜糖分含量与蔗糖代谢酶活性动态变化[J].北方农业学报,2026,54(2):36-47,12.基金项目
宿迁市农业科技自主创新项目(SQCX202409) （SQCX202409）
