农业工程2026，Vol.16Issue(5)：64-71,8.DOI:10.19998/j.cnki.2095-1795.202512016
基于16S rRNA的奶牛乳汁及环境中菌群结构分析
Analysis of microbial community structure in cow milk and environment based on 16S rRNA
摘要
Abstract
To investigate effects and source of pathogens causing bovine mastitis on microbial community in milk,16S rRNA sequen-cing technology was employed.Samples were collected from cows with mastitis and healthy cows at a dairy farm in Yantai City,Shan-dong Province,as well as from milking parlour,cowshed air,and feces.Microbial community structures were compared,and sources were traced using SourceTracker.Results showed that milk of cows with mastitis increased bacterial abundance but reduced diversity.Samples from the same source had similar compositions,while significant differences were observed among samples from healthy cows,mastitis-affected cows and faeces.Moreover,results indicated that Streptococcus uberis was the main pathogen causing mastitis at stud-ied farm,with changes in milk microbiota following mastitis.Source tracking with SourceTracker suggested that pathogens mainly ori-ginated from milking parlor environment.These results provide a theoretical reference for prevention and control of mastitis in dairy cows.关键词
奶牛乳房炎/乳房链球菌/菌群结构/病原菌溯源/16S rRNAKey words
bovine mastitis/Streptococcus uberis/microbial community structure/pathogen traceability/16S rRNA分类
农业科技引用本文复制引用
王凯,宋宗润,王光暄,王淑祺,杨政超,张浩洋,鲍鹏天,南松剑..基于16S rRNA的奶牛乳汁及环境中菌群结构分析[J].农业工程,2026,16(5):64-71,8.基金项目
2023年烟台市校地融合发展项目(2023XDRHXMPT12) （2023XDRHXMPT12）
