热带亚热带植物学报2026，Vol.34Issue(3)：328-336,9.DOI:10.11926/jtsb.5045
3种介质盆栽辣椒根系黄酮类化合物及其合成酶基因影响研究
Effect on Metabolites and Their Synthetases of Flavonoids in Root Under 3 Media Potting Capsicum annuum
摘要
Abstract
To understand the root response mechanism of Capsicum annuum under continuous cropping obstacles,three types of media,namely substrate soil(CSS),rotation soil(CRS)and continuous cropping soil(CCS),were used to pot the peppers.The root systems were collected when they entered the fruiting period for omics research to analyze the specific components of flavonoids and the information of their synthase genes in the root systems of peppers.The results showed that a total of 31 flavonoids were detected in the root systems of peppers in the three types of media.Compared with CSS,the contents of six flavonoids,namely pelargonidin 3-O-caffeoyl-β-D-glucoside,cyanidin 3-O-glucosides(2 types),delphinidin 3-O-β-D-glucoside,neohesperidin,quercetin 3-O-β-D-glucosyl-β-D-glucoside,were significantly increased in the two cultivation conditions of CRS and CCS,while only one flavonoid,malvidin-3-rutinoside-5-glucoside,was significantly decreased(by 82.37%and 91.29%,respectively).The synthesis of flavonoids involves 202 enzyme genes,among which the families with more genes mainly include cytochrome P450(15)and UDP glycosyltransferase(14).Compared with CSS,the expression of main synthase genes of these flavonoids in CRS and CCS were significantly different,including 23 flavonoid synthase genes,3 anthocyanin synthase genes,7 isoflavone synthase genes,and 5 flavone and flavonol synthase genes.Among them,the expression of UDP glycosyltransferase genes in anthocyanin synthesis metabolism(1)and flavone and flavonol synthesis metabolism(3)were significantly increased on CCS cultivation(by 150.00%,161.45%,92.77%and 773.50%,respectively).Therefore,continuous cropping and other adverse stress could significantly increase the content of most flavonoids in the root systems of peppers,and the up and down regulation trends of their synthase genes were not obvious.关键词
辣椒/转录组/代谢组/连作障碍/逆境栽培/次生代谢物Key words
Capsicum annuum/Metabolome/Transcriptome/Continuous cropping barrier/Stress cultivation/Secondary metabolite引用本文复制引用
张少平,李洲,赖正锋,练冬梅,林碧珍,鞠玉栋..3种介质盆栽辣椒根系黄酮类化合物及其合成酶基因影响研究[J].热带亚热带植物学报,2026,34(3):328-336,9.基金项目
福建省公益类科研院所基本科研专项(2021R1030002,2022R1030001,2022R1030002,2023R1028004,2024R1077) （2021R1030002,2022R1030001,2022R1030002,2023R1028004,2024R1077）
福建省农业科学院自由探索科技创新项目(ZYTS202207)资助 This work was supported by the Special Project for Public Welfare Research Institutes in Fujian(Grant No.2021R1030002,2022R1030001,2022R1030002,2023R1028004,2024R1077),and the Project for Freely Explore Scientific and Technological Innovation of Fujian Academy of Agricultural Sciences(Grant No.ZYTS202207). （ZYTS202207）
