热带亚热带植物学报2026，Vol.34Issue(3)：337-345,9.DOI:10.11926/jtsb.5010
姜黄倍半萜合酶基因ClFLDH的克隆和原核表达分析
Cloning and Prokaryotic Expression Analysis of Sesquiterpene Synthase Gene ClFLDH from Curcuma longa
摘要
Abstract
The farnesol dehydrogenase gene(ClFLDH)of Curcuma longa is a key rate-limiting enzyme in the terpene compound synthesis pathway.To explore the improvement of terpene compound production in C.longa,the cDNA sequence of ClFLDH was obtained through homologous cloning.The open reading frame(ORF)of ClFLDH was 855 bp,encoding 295 amino acids.Bioinformatics analysis indicated that the relative molecular mass of ClFLDH protein was 31 kDa,with an isoelectric point of 6.79,and it was a liposoluble,hydrophobic and neutral protein.The pCE32a-FLDH prokaryotic expression vector was constructed and expressed in Escherichia coli DH5α.The SDS-PAGE electrophoresis results of the recombinant strain showed that the size of the target protein was 37 kDa.GC-MS analysis indicated that the ClFLDH protein might catalyze 3,5-dimethoxyphenol to generate[1,2-a]pyrazine-1,4-dione,6H-3-(benzyl)-pyrrole.These would provide a new perspective for in-depth understanding of the regulatory mechanism of curcuminoid terpene synthesis and lay a foundation for increasing the production of curcuminoid terpene compounds through genetic engineering.关键词
姜黄/法尼醇脱氢酶基因/生物信息学/萜烯合酶/原核表达Key words
Curcuma longa/Farnesol dehydrogenase gene/Bioinformatics/Terpene synthase/Prokaryotic expression引用本文复制引用
林洪铭,温思琦,李花,江民超,刘建福,于海玲,王明元,王奇志..姜黄倍半萜合酶基因ClFLDH的克隆和原核表达分析[J].热带亚热带植物学报,2026,34(3):337-345,9.基金项目
福建省科技计划项目(2024N3007)资助 This work was supported by the Project for Science and Technology Plan in Fujian(Grant No.2024N3007). （2024N3007）
