热带作物学报2026，Vol.47Issue(5)：1107-1118,12.DOI:10.3969/j.issn.1000-2561.2026.05.001
菠萝4CL基因家族的鉴定及表达分析
Identification and Expression Analysis of Pineapple 4CL Gene Family
摘要
Abstract
4-coumaric acid:CoA ligase(4CL)gene regulates the biosynthesis of lignin and flavonoids,playing an im-portant role in plant organ color formation,disease resistance,and stress resistance.In order to understand the molecular characteristics and biological functions of the pineapple 4CL gene family,this study used bioinformatics technologies and tools to identify and analyze members of the 4CL gene family in the pineapple genome,followed by experimental validation.A total of 30 4CL genes were identified from the pineapple genome,and the physicochemical properties such as amino acid number,molecular weight,and isoelectric point were variant.Members of the Ac4CL gene family were unevenly distributed on 13 out of 25 chromosomes in pineapple.There were 5 pairs of genes exhibiting intraspecific collinearity,and 6 pairs of genes exhibiting collinearity between pineapple and Arabidopsis.There were significant dif-ferences in the expression of Ac4CL in pineapple at 60,90 and 120 days after flowering.Among them,Ac4CL2 showed increasingly significant differences in expression after flowering.Furthermore,the gene also showed significant differ-ences in expression among four different pineapple varieties,namely Jinzuan,Milk,Watermelon,and Bali,and it is inferred that they are closely related to the accumulation of anthocyanins during fruit ripening.This study would provide a theoretical basis for further exploring the function of the pineapple 4CL gene family.关键词
菠萝/香豆酸:辅酶A连接酶(4CL)/基因家族/抗逆Key words
pineapple/4-coumarate:CoA ligase(4CL)/gene family/stress resistance分类
农业科技引用本文复制引用
邹幸江,段元浩,崔世娇,肖霄,尚永才,冯世鹏,孙伟生..菠萝4CL基因家族的鉴定及表达分析[J].热带作物学报,2026,47(5):1107-1118,12.基金项目
2024年省级乡村振兴战略专项资金种业振兴项目(No.2024-NPY-00-029) （No.2024-NPY-00-029）
海南省重点研发项目(No.ZDYF2021XDNY117). （No.ZDYF2021XDNY117）
