热带作物学报2026，Vol.47Issue(5)：1152-1162,11.DOI:10.3969/j.issn.1000-2561.2026.05.005
槟榔种质资源农艺及品质性状InDel标记关联分析
Association Analysis of Agronomic and Quality Traits with InDel Markers in Arecanut Germplasm Resources
摘要
Abstract
Arecanut(Areca catechu L.)is an important tropical specialty economic crop in Hainan Province.It is listed as the first of the four south China medicinal plants and holds a significant position in Hainan's agricultural production.Uncovering molecular markers associated with arecanut agronomic and quality traits has great significance for breeding new high-quality varieties with desirable traits,and molecular marker-assisted breeding.In the research,50 pairs of InDel markers were utilized to genotyping 149 arecanut germplasm resources,and the genetic diversity and population structure analysis were also conducted.The association analysis was performed using both the GLM and MLM associa-tion analysis models to identify the molecular markers associated with phenotypic traits.The results showed a total of 123 allele loci were detected in the population.The average observed heterozygosity(Ho)and expected heterozygosity(He)was 0.36 and 0.43 respectively.The average Shannon's information index(I)was 1.1548,and the polymorphism information content(PIC)of the primers was ranged from 0.15 to 0.52,with an average of 0.35.88.00%of the primers exhibited moderate to high polymorphism,which indicating that the InDel primers in this study had high polymorphism.The population structure analysis suggested that 149 arecanut divided into three subgroups,which consisting of 118,24 and 7 materials respectively.Using the GLM and MLM association analysis models,58 and 40 associated loci were detected,with phenotypic variation explanation rates ranging from 4.36%to 13.39%and 4.35%to 9.29%respectively.All associated loci detected by the MLM model were also detected by the GLM model.The markers AC1505 and AC1519 were detected to be associated with fruit shape and quality-related traits in both methods,exhibiting a signifi-cant"pleiotropic effects"phenomenon.The findings would provide a theoretical basis for gene mining related to are-canut traits and breeding for quality characteristics.关键词
槟榔/农艺及品质性状/InDel标记/关联分析Key words
arecanut/agronomic and quality traits/InDel markers/association analysis分类
农业科技引用本文复制引用
张雨洁,钟木兰,黄丽云,叶剑秋,齐兰..槟榔种质资源农艺及品质性状InDel标记关联分析[J].热带作物学报,2026,47(5):1152-1162,11.基金项目
海南省科技专项(No.ZDYF2022XDNY205) （No.ZDYF2022XDNY205）
海南省槟榔产业技术体系项目(No.HNARS-1-G2) （No.HNARS-1-G2）
农业农村部作物种质资源保护项目. （）
