低渗诱导菲律宾蛤仔三倍体效果评估
Evaluation of the efficiency of triploid production in Manila clam(Ruditapes philippinarum)via hypotonic induction
摘要
Abstract
The Manila clam(Ruditapes philippinarum),one of the most economically significant maricultured bivalves in China,faces critical challenges in genetic improvement due to the scarcity of high-quality breeding stocks.Triploid induction,a proven strategy for enhancing growth and survival in aquatic species,has yet to be systematically optimized for this species.While conventional methods have been applied in bivalve polyploidy induction,their efficiency and safety remain suboptimal.To determine the optimal conditions for triploid induction in R.philippinarum using a hypotonic method,an orthogonal experi-mental design was employed to evaluate the effects of salinity(6,8,10,12,14,16),induction timing(initiation of the first polar body extrusion,the time when 50%of the first polar body are extruded,and initiation of the second polar body extrusion),and induction duration(10,15,20 min)under controlled conditions[water temperature:(25±0.5)℃;pH:8.3±0.1].Develop-mental metrics,including malformation rate,hatching rate,and triploid induction rate of D larvae were quantified.The treated larvae were cultivated in filtered seawater for one month and the survival rates were assessed across different experimental groups.The results demonstrated that the optimal induction protocol involved treating fertilized eggs with low-salinity seawa-ter(salinity=16)for 15 min after 50%of the fertilized eggs had extruded the first polar body.Malformation rate,hatching rate,and triploid induction efficiency were significantly influenced by salinity.Notably,the survival rates of larvae at early stages in the control group were significantly higher than those in the induced groups.This study showed that the hypotonic method enabled efficient triploid induction in R.philippinarum through combinatorially regulating salinity,induction duration,and crit-ical developmental timing.The chemical inducers-associated toxicity could therefore be avoided and the triploid induction cost could be reduced.This study for the first time reported the triploid R.philippinarum production via hypotonic induction,laying a foundation for improving artificial triploid induction technologies in bivalve aquaculture.关键词
菲律宾蛤仔/正交实验/低渗/三倍体Key words
Ruditapes philippinarum/orthogonal experimental/hypotonic induction/triploidy分类
农业科技引用本文复制引用
罗星红,白伟凝,李博睿,崔士汛,孙宇,李征远,张刚,霍忠明,闫喜武..低渗诱导菲律宾蛤仔三倍体效果评估[J].水产学报,2026,50(7):32-44,13.基金项目
辽宁省科技重大专项(2024JH1/11700010) （2024JH1/11700010）
山东省重点研发计划(2023LZGCQY001) （2023LZGCQY001）
辽宁省渔业补贴专项(2024,2025) （2024,2025）
辽宁省科技厅,辽宁省科技计划联合计划(2024JH2/102600078) （2024JH2/102600078）
国家贝类产业技术体系(CARS-49) （CARS-49）
辽宁省"兴辽英才"青年拔尖人才项目(XLYC1807271) （XLYC1807271）
大连市杰出青年科技人才项目(2021RJ09) （2021RJ09）
国家自然科学基金(32573502) Liaoning Provincial Major Science and Technology Special Project(2024JH1/11700010) （32573502）
Key R&D pro-gram of Shandong Province(2023LZGCQY001) （2023LZGCQY001）
Central Government Subsidy Project for Liaoning Fisheries(2024,2025) （2024,2025）
Liaoning Provincial Department of Science and Technology,Joint Program of Liaoning Provincial Science and Technology Program(2024JH2/102600078) （2024JH2/102600078）
Modern Agro-industry Technology Research System(CARS-49) （CARS-49）
Liaoning Revitalization Tal-ents Program(XLYC1807271) （XLYC1807271）
Outstanding Young Scientific and Technological Talents Foundation of Dalian(2021RJ09) （2021RJ09）
National Natural Science Foundation of China(32573502) （32573502）
