水产学报2026，Vol.50Issue(7)：45-56,12.DOI:10.11964/jfc.20251215245
促性腺激素释放激素(GnRHs)在大菱鲆卵巢不同发育时期的基因表达
Expression of GnRHs during ovarian development in turbot(Scophthalmus maximus)
摘要
Abstract
The turbot(Scophthalmus maximus)is a marine fish of high commercial value and widely cultured in Europe and China.Gonadotropin-releasing hormones(GnRHs),as a neuropeptide of sex hormone,stimulate synthesis and release of pituit-ary gonadotropins,inducing vertebrate gonads to provide sex steroids for the formation and maturation of gametes.Hence GnRH has been proven to be the key signal molecule in the hypothalamus-pituitary-gonadal axis.However,their dynamics expression and functional roles during the ovarian developmental in S.maximus remained not fully elucidated.The present study aimed to investigate the expression patterns and key subtype localization of GnRHs in the brain of turbot during ovarian developmental.Ovarian developmental stages and brain structures were identified using hematoxylin and eosin(H.E)staining.The mRNA levels of gnrhs in various brain regions during ovarian developmental were detected by quantitative real-time PCR(qRT-PCR).The structure of S.maximus brain exhibited a regular linear arrangement of the telencephalon,mesencephalon,cerebellum,hypothalamus,and medulla oblongata,with highly differentiated functions and the mesencephalon occupying the largest proportion.Ovarian development obtained the typical pre-vitellogenic,early-vitellogenic,late-vitellogenic,nuclear migration,and atresia stages.The mRNA levels of gnrh3 was peaked at the late-vitellogenic stage,while gnrh1 and gnrh2 were peaked at the nuclear migration stage.Meanwhlie,the mRNA levels of gnrh1 in all brain regions were significantly higher than those of other subtypes,the highest values were observed in the telencephalon at the nuclear migration stage.In situ hybridiza-tion further confirmed that gnrh1-positive signals were enriched in the ventral telencephalon and hypothalamus.Thus,the large relative area of the S.maximus mesencephalon confers enhanced visual information processing capacity,enabling adaptation to complex benthic environments.Moreover,the heightened expression of gnrh1 specifically in the telencephalon during the nuc-lear migration regulates the final maturation of oocytes,identifying it as the key subtype regulating ovarian development and maturation in S.maximus.This study systematically delineates the brain architecture of the S.maximus and uncovers the expression profiles of GnRH subtypes throughout ovarian development.These findings provide a crucial theoretical foundation for investigating neuroendocrine regulatory mechanisms and for the development of efficient reproductive management strategies in captivity.关键词
大菱鲆/GnRHs/基因表达/脑/卵巢Key words
Scophthalmus maximus/GnRHs/gene expression/brain/ovary分类
生物科学引用本文复制引用
张楠,宋嘉玲,贾玉东..促性腺激素释放激素(GnRHs)在大菱鲆卵巢不同发育时期的基因表达[J].水产学报,2026,50(7):45-56,12.基金项目
国家自然科学基金(32473184) （32473184）
中国水产科学研究院科技创新团队项目(2023TD81) National Natural Science Foundation of China(32473184) （2023TD81）
Science and Technology Innovation Team Project of Chinese Academy of Fishery Sciences(2023TD81) （2023TD81）
