四川医学2026，Vol.47Issue(5)：509-515,7.DOI:10.16252/j.cnki.issn1004-0501-2026.05.006
LncRNA SOX2-OT调节miR-194-5p/SRXN1轴对食管鳞状细胞癌细胞发生发展的影响
Effect of LncRNA SOX2-OT on the Occurrence and Development of Esophageal Squamous Cell Carcinoma Cells by Regulating the miR-194-5p/SRXN1 Axis
摘要
Abstract
Objective To investigate effects of long non coding RNA(LncRNA)sex determining region Y-box 2 overlap-ping transcript(SOX2-OT)on the occurrence and development of esophageal squamous cell carcinoma(ESCC)cells by regula-ting the microRNA-194-5p(miR-194-5p)/thioredoxin 1(SRXN1)axis.Methods Human ESCC TE-1 cells were grouped into Control group(normal culture),sh-NC group(transfected with sh-NC),sh-SOX2-OT group(transfected with sh-SOX2-OT),sh-SOX2-OT+anti-NC group(transfected with sh-SOX2-OT+anti-NC),and sh-SOX2-OT+anti-miR-194-5p group(transfected with sh-SOX2-OT+anti-miR-194-5p).QRT-PCR method was applied to detect mRNA expression of LncRNA SOX2-OT,miR-194-5p,and SRXN1 in various TE-1 cells.EdU staining,Transwell assay,and flow cytometry were used to measure the prolifera-tion,migration,invasion,and apoptosis of TE-1 cells in each group.Western Blot was applied to detect the expression of PCNA,SRXN1,MMP-9,and Cleaved Caspase-3 proteins in TE-1 cells.Dual luciferase reporter gene assay was applied to detect the inter-action between LncRNA SOX2-OT and miR-194-5p,and between miR-194-5p and SRXN1.Results The EdU positive cell rate,migration number,invasion number,LncRNA SOX2-OT and SRXN1 mRNAs and proteins,PCNA,SRXN1,and MMP-9 proteins in the sh-SOX2-OT group were lower than those in sh-NC group and control group,apoptosis rate,miR-194-5p,and cleaved Caspase-3 protein expression were higher in sh-NC group and control group(P＜0.05).Compared with sh-SOX2-OT group and sh-SOX2-OT+anti-NC group,the sh-SOX2-OT+anti-miR-194-5p group showed an increase in EdU positive cell rate,migration num-ber,invasion number,SRXN1 mRNA and protein,PCNA,SRXN1,MMP-9 protein expression,and a decrease in apoptosis rate,miR-194-5p,and cleaved Caspase-3 protein expression(P＜0.05).LncRNA SOX2-OT was able to target negative regulation of miR-194-5p,miR-194-5p was able to target negative regulation of SRXN1.Conclusion Knockout of LncRNA SOX2-OT could in-hibit the occurrence and development of ESCC cells,and its mechanism may be achieved by regulating the miR-194-5p/SRXN1 signaling pathway.关键词
长链非编码RNA/性别决定相关基因簇2重叠转录本/微小RNA-194-5p/硫氧还蛋白-1/食管鳞状细胞癌Key words
LncRNA/sex determining region Y-box 2 overlapping transcript/miRNA-194-5p/thioredoxin 1/esophageal squamous cell carcinoma分类
医药卫生引用本文复制引用
郭剑锋,熊玉梅,许芬芬,刘劼..LncRNA SOX2-OT调节miR-194-5p/SRXN1轴对食管鳞状细胞癌细胞发生发展的影响[J].四川医学,2026,47(5):509-515,7.基金项目
攀枝花市2022年度市级指导性科技计划项目(编号:2022ZD-S-48) （编号:2022ZD-S-48）
