摘要
Abstract
Objective To investigate expression changes of serum microRNA-204-5p(miR-204-5p)and cysteine-rich protein 61(CCN1)in patients with psoriasis vulgaris(PV),and their relationship with immune inflammatory response.Methods 80 PV patients who visited our hospital between August 2022 and August 2024 and met research criteria were selected as study subjects.They were assigned into a mild group of 44 patients and a severe group of 36 patients based on their disease status.Addi-tionally,60 healthy individuals who underwent physical examinations at our hospital during the same period were selected as the control group.RT-PCR method was applied to measure the expression of miR-204-5p in serum.ELISA experiment was applied to detect the expression of CCN1 in serum.Pearson correlation was applied to analyze the relationship between miR-204-5p and CCN1 expression in serum and immune inflammation related indicators.Logistic regression was applied to analyze the impact of immune inflammation related factors on the occurrence of PV.Results The miR-204-5p in serum mild PV group and severe PV group was significantly lower than that of control group,and severe PV group was lower than mild PV group(P＜0.05).The CCN1 in serum mild PV group and severe PV group was significantly higher than that of control group,and severe PV group was higher than mild PV group(P＜0.05).The ESR,neutrophil count,Th1/Th2,IL-2,INF-γ,and hs-CRP levels in mild PV group and severe PV group were significantly higher than those in control group,and severe PV group was higher than mild PV group(P＜0.05).IL-4 and IL-10 in mild PV group and severe PV group were significantly lower than those in control group,and severe PV group was low-er than mild PV group(P＜0.05).The PASI,DLQI and BSA score in severe PV group were significantly higher than those in mild PV group(P＜0.05).Serum miR-204-5p was negatively correlated with ESR,neutrophil count,Th1/Th2,IL-2,INF-γ,hs-CRP,and positively correlated with IL-4 and IL-10(P＜0.05).Serum CCN1 was positively correlated with ESR,neutrophil count,Th1/Th2,IL-2,INF-γ,hs-CRP,and negatively correlated with IL-4 and IL-10(P＜0.05).Th1/Th2,IL-2,INF-γ,IL-4,IL-10,miR-204-5p,and CCN1 were risk factors of PV occurrence(P＜0.05).Conclusion The miR-204-5p in PV patients is decreased,while CCN1 is increased.The expression levels of both are closely related to the severity of PV and inflammatory response of PV patients.关键词
寻常型银屑病/微小RNA-204-5p/富含半胱氨酸的蛋白61/免疫炎症反应Key words
psoriasis vulgaris/microRNA-204-5p/cysteine-rich protein 61/immune inflammatory response分类
医药卫生
