上海预防医学2026，Vol.38Issue(3)：216-220,5.DOI:10.19428/j.cnki.sjpm.2026.250315
杭州市拱墅区一起人偏肺病毒疫情的分子特征分析
Molecular characterization analyses of a human metapneumovirus outbreak in Gongshu District of Hangzhou City
摘要
Abstract
[Objective]To analyze the epidemiological and etiological characteristics of a cluster of human metapneumovirus(HMPV)infection in a kindergarten in Gongshu District of Hangzhou City in May 2024,and to provide reference for the prevention and control of similar outbreaks.[Methods]An on-site investigation was conducted using an epidemiological case investigation form.Throat swab specimens collected from cases were screened for 13 respiratory pathogens using real-time fluorescent polymerase chain reaction(PCR).For HMPV nucleic acid positive specimens,the F gene of HMPV was used as the target gene for amplification and sequencing.The sequencing results were then compared with sequences in GenBank database to determine the virus subtypes and perform phylogenetic analyses.[Results]The outbreak occurred in a kindergarter junior class with a total of 28 preschoolers and 3 teachers and childcare workers.A total of 11 cases(10 preschoolers and 1 teacher)were identified,including 8 male cases and 3 female cases.Clinical manifestations included fever in all 11 cases(100.00%),cough in 8 cases(72.72%),catarrhal symptoms in 4 cases(36.36%),and headache in 3 cases(27.27%).All symptoms were mild,and no severe cases were observed.A total of 11 throat swab samples were collected.Real-time fluorescent PCR test results showed that 3 samples were positive for HMPV nucleic acid,2 samples were positive for both HMPV and Streptococcus pneumoniae,and 1 sample was positive for both HMPV and rhinovirus.The sequences of the 6 HMPV nucleic acid positive specimens were amplified and analyzed using specific primers,and all were determined to be HMPV subtype A2b.The F gene fragment sequence showed the highest similarity to PV081665.1/Brazil/2024(99.65%),and also exhibited high similarity to PP683455.1/Indonesia/2021(99.48%),PV016275.1/Beijing/2024(99.31%),and PV052230.1/USA/2024(99.13%).[Conclusion]This cluster of acute respiratory tract infection was caused by HMPV subtype A2b,with co-infection of rhinovirus and Streptococcus pneumoniae.The F gene fragment sequences of the HMPV in this outbreak were highly homologous to those of the A2b strains isolated from Brazil,Beijing,Indonesia,and the the United States.关键词
人偏肺病毒/呼吸道感染/分子特征/进化树分析Key words
human metapneumovirus/respiratory tract infection/molecular characterization/phylogenetic tree analysis分类
医药卫生引用本文复制引用
刘健毅,张晨烨,赵磊,帅慧群,郁欢欢,孙晴雨,鲁飞,席胜军..杭州市拱墅区一起人偏肺病毒疫情的分子特征分析[J].上海预防医学,2026,38(3):216-220,5.基金项目
浙江省医药卫生科技计划项目(2023XY120) （2023XY120）
