上海预防医学2026，Vol.38Issue(4)：289-295,7.DOI:10.19428/j.cnki.sjpm.2026.250389
2023-2024年上海市宝山区腹泻病沙门菌血清型及耐药性分析
Analyses of serotypes and antibiotic resistance of Salmonella in diarrheal diseases in Baoshan District of Shanghai from 2023 to 2024
摘要
Abstract
[Objective]To retrospectively analyze the correlation among serotypes,antimicrobial resistance phenotypes,and resistance genotypes of Salmonella isolates from diarrheal cases in Baoshan District of Shanghai from 2023 to 2024,and to provide a reference for the prevention and control of Salmonella infections and the rational use of antibiotics in clinical practice.[Methods]Salmonella isolates collected from diarrheal cases under surveillance in Baoshan District from 2023 to 2024 were serotyped.Antimicrobial resistance phenotypes were determined using the broth microdilution method,and whole-genome sequencing was performed to identify resistance genes.Positive predictive value and Kappa were calculated to evaluate the agreement between phenotypic and genotypic resistance profiles of Salmonella.[Results]A total of 64 Salmonella isolates belonged to 17 serotypes,with the predominant ones being Salmonella Typhimurium(25.00%)and Salmonella Enteritidis(18.75%).The tested strains exhibited high resistance rates to ampicillin(60.94%),streptomycin(59.38%),and ampicillin/sulbactam(45.31%).All isolates remained susceptible to ceftiofur and ceftazidime/avibactam.Forty different resistance profiles were identified,and 39 isolates accounting for 60.94%,were multidrug-resistant.Overall,80 resistance genes belonging to 13 categories were detected,with the most prevalent being blaTEM-1(57.81%),aac(6')-Iy(54.69%),and aph(6)-Id(46.88%).No carbapenem or polymyxin resistance genes were found.The types and numbers of resistance genes varied significantly across serotypes.A high concordance was observed between genotype and phenotype for penicillins(positive predictive value:94.59%,Kappa:0.81),cephalosporins(90.91%,0.46),cephamycins(100.00%,0.65),macrolides(77.78%,0.86),tetracyclines(100.00%,0.93),and chloramphenicol(95.83%,0.93).[Conclusion]Salmonella isolates from diarrheal diseases in Baoshan District exhibit high antimicrobial resistance.Whole-genome sequencing provides valuable support for resistance surveillance,however,it still needs to be integrated with phenotypic susceptibility testing for comprehensive assessment.Further studies are warranted to elucidate the mechanisms and transmission patterns of resistance in Salmonella.关键词
沙门菌/沙门菌血清型/抗生素耐药性/多重耐药/全基因组测序/耐药基因Key words
Salmonella/Salmonella serotype/antibiotic resistance/multidrug resistance/whole-genome sequencing/resistance gene分类
医药卫生引用本文复制引用
林芳甸,张旭,沈隽卿,金敏..2023-2024年上海市宝山区腹泻病沙门菌血清型及耐药性分析[J].上海预防医学,2026,38(4):289-295,7.基金项目
上海市加强公共卫生体系建设三年行动计划(2023-2025年)学科人才建设项目(GWVI-11.1-06) （2023-2025年）
上海市宝山区医学重点专科A类项目(BSZK-2023-A20) （BSZK-2023-A20）
