世界科学技术-中医药现代化2026，Vol.28Issue(5)：1358-1369,12.DOI:10.11842/wst.20251226006
肿瘤三维细胞球与类器官代谢物质谱成像研究进展
Research Progress on Mass Spectrometry Imaging of Metabolites in 3D Tumor Spheroids and Organoids
摘要
Abstract
Three-dimensional tumor spheroids and organoids can effectively simulate the spatial heterogeneity and microenvironmental characteristics of tumors in vivo,and have emerged as important models in tumor metabolism research.Mass spectrometry imaging(MSI)technology enables in situ analysis of the distribution of metabolites,lipids,and drugs while preserving the spatial information of tissues.The integration of three-dimensional tumor models and mass spectrometry imaging allows for the precise analysis of tumor metabolic reprogramming and its spatial heterogeneity.This review systematically summarizes the methods for relative model construction and the sample preparation strategies tailored for MSI.It also introduces the core techniques features of MSI,with a particular focus on its applications progress in combination with three-dimensional tumor models for elucidating spatial metabolic heterogeneity characteristics of tumors,molecular mechanism,and the spatial distribution of drugs.This work aims to provide methodological guidance and theoretical support for studies on tumor metabolic mechanisms and drug development.关键词
肿瘤三维细胞球/类器官/质谱成像/空间代谢异质性Key words
Tumor three-dimensional spheroids/Organoids/Mass spectrometry imaging/Spatial metabolic heterogeneity分类
医药卫生引用本文复制引用
张秀童,王婕雅,张雅琪,陈世平,王晓,陈盼盼,孙成龙..肿瘤三维细胞球与类器官代谢物质谱成像研究进展[J].世界科学技术-中医药现代化,2026,28(5):1358-1369,12.基金项目
国家自然科学基金委员会面上项目(82273888),基于整合式空间分辨多组学的乳腺癌肿瘤代谢靶点识别新方法研究,负责人:孙成龙 （82273888）
国家自然科学基金青年项目(82404587),基于细胞特异性空间代谢组学的肿瘤免疫耐药机制研究,负责人:陈盼盼 （82404587）
云南省教育厅科学研究基金项目(2024J0395),利伐沙班在静脉血栓栓塞症治疗中的真实世界研究,负责人:王婕雅. （2024J0395）
