世界科学技术-中医药现代化2026，Vol.28Issue(5)：1377-1389,13.DOI:10.11842/wst.20260119003
酸枣仁汤调控慢性睡眠剥夺大鼠脑微区空间代谢网络作用研究
To Explore the Spatial Metabolic Networks in Sleep-Deprived Rat Brain Microregions And Regulation Effects of Suanzaoren Decoction
摘要
Abstract
Objective To elucidate the mechanism by which Suanzaoren Decoction(SZRD)exerts its anti-insomnia and anxiolytic-like effects by in situ visualization of spatial metabolic networks in specific brain microregions of sleep-deprived rats,leveraging the data dimensionality reduction features of metabolomics and mass spectrometry imaging(MSI)technology.Methods A modified multiple-platform water environment rat model of sleep deprivation was established.The therapeutic efficacy of SZRD was evaluated using the elevated plus maze test and serum corticosterone levels.MSI technology was employed to observe the in situ expression and trends of small-molecule metabolites in the brains of sleep-deprived rats and to investigate the intervention characteristics of SZRD.Results After 21 days of sleep deprivation using the modified multiple-platform method,normal rats exhibited anxiety-like behaviors.Compared to the model group,SZRD significantly increased the time ratio and number of entries into the open arms(P<0.05),reduced serum corticosterone levels(P<0.01),and markedly ameliorated insomnia and anxiety-like behaviors in sleep-deprived rats.MSI results showed that chronic sleep deprivation significantly altered the levels of neurotransmitters and their metabolites in the hippocampus,hypothalamus,striatum,and cortex of rats.SZRD significantly modulated the levels of 34 differential metabolites in the brain,primarily including amino acids and their metabolites.Pathway analysis suggested that SZRD may exert its effects by regulating histidine metabolism,alanine,aspartate and glutamate metabolism,tryptophan metabolism,tyrosine metabolism,and arginine metabolism pathways.Conclusion SZRD exerts anti-insomnia and anxiolytic effects by regulating small-molecule amino acid metabolic pathways(such as tryptophan and tyrosine metabolism)and modulating the content and spatial distribution of neurotransmitters in the brain.These findings provide a scientific basis for the precise application and multi-target characteristics of SZRD in improving sleep disorders.关键词
酸枣仁汤/睡眠剥夺/质谱成像/空间代谢组学/氨基酸代谢Key words
Suanzaoren Decoction/Sleep deprivation/Mass spectrometry imaging/Spatial metabolomics/Amino acid metabolism分类
医药卫生引用本文复制引用
闫艳,孙亚禹,陶宇,刘嘉颖,杜晨晖,张瑞平..酸枣仁汤调控慢性睡眠剥夺大鼠脑微区空间代谢网络作用研究[J].世界科学技术-中医药现代化,2026,28(5):1377-1389,13.基金项目
山西省中医药管理局科研项目(2024ZYYB028):基于空间代谢组学的酸枣仁调控"脑-肠"组织微区发挥养心安神作用机制研究,负责人:闫艳. （2024ZYYB028）
