世界科学技术-中医药现代化2026，Vol.28Issue(5)：1535-1545,11.DOI:10.11842/wst.20250519005
基于转录组学探讨天麻乙酸乙酯提取物治疗肌少症的作用机制
Transcriptomics-based Analysis of the Mechanism of Action of the EtOAc Extract from Gastrodia elata in the Treatment of Sarcopenia
摘要
Abstract
Objective Based on transcriptomics,Caenorhabditis elegans was used to replicate a dexamethasone-induced sarcopenia model to explore the preventive and therapeutic effects of EtOAc extract from Gastrodia elata(EEGE)on sarcopenia.Methods L4-stage N2 worms were placed on corresponding experimental plates to complete drug administration and model replication.Muscle function was detected by swimming and crawling tests,and muscle state was observed by phalloidin staining.Reactive oxygen species(ROS)fluorescence staining,nitric oxide(NO)content,antioxidant enzyme activity,and total antioxidant capacity were measured to reflect the level of oxidative stress.Transcriptomics and PCR-based microarrays were used to screen for possible mechanisms of EEGE in treating sarcopenia.Results Compared with the model group,EEGE treatment significantly increased swimming and crawling frequencies(P<0.01,P<0.001).After EEGE treatment,the body wall muscle stripes of the worms were clear and regularly arranged.EEGE effectively combated oxidative stress damage,significantly reducing ROS and NO levels in the worms(P<0.001)while increasing antioxidant enzyme activity and total antioxidant capacity(P<0.05,P<0.001).Transcriptomics analysis suggested that EEGE may alleviate sarcopenia by promoting autophagy,upregulating glutathione metabolism-related pathways,and regulating biological processes such as the cell matrix and cuticle.Conclusion The results indicate that EEGE significantly improves muscle function and state in worms,and has potential therapeutic effects on sarcopenia.关键词
肌少症/天麻/乙酸乙酯提取物/秀丽隐杆线虫/衰老/氧化应激/自噬Key words
Sarcopenia/Gastrodia elata/EtOAc extract/Caenorhabditis elegans/Senescence/Oxidative stress/Autophagy分类
医药卫生引用本文复制引用
肖湉,于兴志,陶婕,杨丽萍,张超,段小花..基于转录组学探讨天麻乙酸乙酯提取物治疗肌少症的作用机制[J].世界科学技术-中医药现代化,2026,28(5):1535-1545,11.基金项目
云南省傣医药与彝医药重点实验室开放项目(2021KY005):彝药天麻提取物对地塞米松诱导肌少症小鼠模型的影响,负责人:肖湉 （2021KY005）
云南中医药大学高层次人才第五层次新锐人才(2023-05-007),负责人:段小花 （2023-05-007）
云南省"兴滇英才支持计划"青年人才(XDYC-QNRC-2022-0284),负责人:段小花. （XDYC-QNRC-2022-0284）
