中国森林病虫2026，Vol.45Issue(3)：24-33,10.DOI:10.19688/j.cnki.issn1671-0886.20260014
两种玛草蛉的线粒体基因组全序列分析及草蛉科系统发育
Complete mitochondrial genome sequence analysis of two species of Mallada and phylogeny of Chrysopidae
摘要
Abstract
To clarify the complete mitochondrial genome(mitogenome)and systematic status of Mallada Navás,1925,the complete mitogenome of Mallada flavimaculus and Mallada viridianus were newly sequenced and annotated by Illumina second-generation sequencing technology.Furthermore,the characteristics of mitogenomes and the phylogenetic relationships within the family Chrysopidae were comparatively analyzed.The result showed that the complete mitogenome of M.flavimaculus and M.viridianus were 16 204 bp and 15 810 bp long,respectively.The AT content of the mitogenome of M.flavimaculus and M.viridianus were 80.14%and 79.16%.ATN was the mostly used start codon among the PCGs of mitogenome in Chrysopidae.Most PCGs used TAA as stop codons,while some PCGs terminated with TA-tRNA or T-tRNA.COX1 was the most conserved gene in the mitogenome of Chrysopidae,while ATP8 was a rapidly evolved gene.Almost all tRNAs could be folded into clover leaf structures,with the exception of tRNASer(AGN).Within Chrysopidae,Apochrysinae were recovered as the sister group to Chrysopinae+Nothochrysinae.Within Chrysopinae,Nothancylini were recovered as the sister group to(Leucochrysini+Belonopterygini)+(Ankylopterygini+(Nineta+Chrysopini)).Mallada were recovered as the sister group to Chrysoperla.These findings not only contribute foundational data for molecular systematic studies in Chrysopidae but also aid in elucidating the differentiation patterns of Chrysopidae mitogenomes.关键词
草蛉科/玛草蛉属/黄斑玛草蛉/绿玛草蛉/线粒体基因组/系统发育Key words
Chrysopidae/Mallada/Mallada flavimaculus/Mallada viridianus/mitochondrial genome/phylogeny分类
农业科技引用本文复制引用
宋振刚,张茹悦,田硕,王玉玉..两种玛草蛉的线粒体基因组全序列分析及草蛉科系统发育[J].中国森林病虫,2026,45(3):24-33,10.基金项目
国家自然科学基金"脉翅目高级阶元系统发育研究——基于低覆盖度的全基因组数据"(32170451),"基于全线粒体基因组的草蛉总科高级阶元系统发育及演化"(31900341) （32170451）
河北省石家庄市高校基础研究项目"基于低覆盖全基因组的褐蛉科高级阶元系统发育及演化"(241791137A) （241791137A）
保定市科技计划项目"基于低覆盖全基因组的中国草蛉亚科系统发育研究"(2472P018) （2472P018）
