饲料研究2026，Vol.49Issue(8)：1-7,7.DOI:10.13557/j.cnki.issn1002-2813.2026.08.001
饲喂干白酒糟对关岭杂交牛肠道菌群结构和代谢谱的影响
Effects of dietary dried distiller's grains on intestinal flora structure and metabolic profile of Guanling crossbred cattle
摘要
Abstract
The experiment aimed to investigate the effects of dried distiller's grains on the intestinal flora and metabolic profile of Guanling crossbred cattle.Twelve healthy Guanling crossbred cattle(Guanling yellow cattle×Simmental cattle)were randomly divided into two groups,with six replicates per group and one cattle per replicate.The control group was fed a basal diet,while the replacement group was fed a diet in which 25%of the concentrate was replaced with dried distiller's grains.The pre-test period was 15 days,and the formal test period was 60 days.The results showed that compared with the control group,the replacement group exhibited an increased relative abundance of Actinobacteriota and a decreased relative abundance of Bacteroidota,while the relative abundances of Firmicutes and Proteobacteria were increased.In the replacement group,the relative abundance of the hgcI clade,Lactobacillus and Prevotellaceae NK3B31 group were increased,and the relative abundance of Bacteroides was decreased.Linear discriminant analysis effect size(LEfSe)analysis revealed that α Proteobacteria and Actinobacteria-relate d gener a ha d th e highes t LD A score s i n th e replacement group,whereas the control group was dominated by Bacteroidetes-related genera.Metabolomics analysis identified 14 differential metabolites,among which 12 metabolites,including sphingosine,taurocholic acid,lithocholyltaurine,and indole-3-ethanol,were significantly up-regulated,while L-tyrosine and cinnamic acid were down-regulated.These metabolites were enriched in 16 pathways,including primary bile acid biosynthesis,bile secretion,and taurine and hypotaurine metabolism.Correlation analysis showed that genera such as C39,Ruminococcus_UCG-014,and hgcI clade were positively correlated with metabolites including taurocholic acid and sphingosine.The study shows that dietary supplementation with dried distiller's grains may positively affect the growth,fattening,and intestinal health of Guanling crossbred cattle by regulating Firmicutes,Proteobacteria,Actinobacteria,and related metabolites,thereby participating in fiber degradation,lipid metabolism,intestinal homeostasis,and anti-inflammatory and antioxidant processes.关键词
干白酒糟/关岭杂交牛/肠道菌群结构/差异代谢物Key words
dried distiller's grains/Guanling crossbred cattle/intestinal flora structure/differential metabolites分类
农业科技引用本文复制引用
张青艳,张天天,朱二鹏,周碧君,杨颖,程振涛..饲喂干白酒糟对关岭杂交牛肠道菌群结构和代谢谱的影响[J].饲料研究,2026,49(8):1-7,7.基金项目
贵州省优秀青年科技人才项目(项目编号:黔科合平台人才[2021]5646号) （项目编号:黔科合平台人才[2021]5646号）
贵州省科技计划项目(项目编号:黔科合基础-ZK[2022]一般158) （项目编号:黔科合基础-ZK[2022]一般158）
