水生生物学报2026，Vol.50Issue(6)：1-10,10.DOI:10.3724/1000-3207.2025.2025.0448
豇豆粉替代豆粕对斑点叉尾鮰生长、肠道结构、肝脏健康及氨基酸代谢的影响
DIETARY SUBSTITUTION OF SOYBEAN MEAL WITH RED COWPEA MEAL ON GROWTH PERFORMANCE,INTESTINAL MORPHOLOGY,LIVER HEALTH,AND AMINO ACID METABOLISM IN CHANNEL CATFISH(ICTALURUS PUNCTATUS)
摘要
Abstract
This study evaluated the effects of replacing soybean meal(SBM)with cowpea meal(CM)on growth perfor-mance,intestinal morphology,liver health,and amino acid metabolism in channel catfish(Ictalurus punctatus).Four isonitrogenous(37%crude protein)and isolipidic(8%crude lipid)diets were formulated,in which CM replaced 0,10%,20%,and 30%of SBM,designated as CON,R10,R20,and R30,respectively.A total of 240 juvenile channel catfish[initial body weight:(22.44±0.28)g]were randomly distributed into 4 groups with 3 replicates each and fed with the experimental diets for 8 weeks.The results showed that the weight gain rate of the R10 and R20 groups was significantly higher than that of the CON group.The specific growth rate of each substitution group was significantly higher than that of the CON group.However,Feed intake in the R10 group was significantly higher than that in the other groups(P<0.05),and a dose-dependent decrease in feed intake was observed with the increasing CM substitution levels.Histopathological examination of intestinal sections stained with hematoxylin and eosin(H&E)showed that dietary CM supplementation significantly increased the goblet cells to villus height ratio,villus height,and muscle layer thickness in the R20 group was significantly higher than that in the CON group(P<0.05).In contrast,H&E and TUNEL staining of liver tissues revealed no noticeable signs of inflammation or apoptosis.Moreover,the transcrip-tional expressions of protein synthesis-related genes(mtor,p70s6k,eif2b)exhibited an upregulation trend.In summary,replacing up to 30%of dietary SBM with red cowpea meal did not impair growth performance or liver protein metabolism in channel catfish,while it improved intestinal morphological structure.关键词
豇豆粉/肠道结构/肝脏健康/氨基酸代谢/斑点叉尾鮰Key words
Cowpea meal/Intestinal morphology/Liver health/Amino acid metabolism/Ictalurus punctatus分类
农业科技引用本文复制引用
韩国立,解绶启,肖彩玲,曹梦西,贾景鲁,张志敏,刘昊昆,金俊琰,梁勇,韩冬..豇豆粉替代豆粕对斑点叉尾鮰生长、肠道结构、肝脏健康及氨基酸代谢的影响[J].水生生物学报,2026,50(6):1-10,10.基金项目
国家重点研发计划(2023YFD2400605) （2023YFD2400605）
国家特色淡水鱼产业技术体系(CARS-46) （CARS-46）
湖北省重点研发项目(2023BBB067)资助[Supported by National Key R&D Program of China(2023YFD2400605) （2023BBB067）
the Earmarked Fund for China Agriculture Research System(CARS-46) （CARS-46）
the Key R&D Project of Hubei Province(2023BBB067)] （2023BBB067）
