水生生物学报2026，Vol.50Issue(6)：11-23,13.DOI:10.3724/1000-3207.2025.2025.0402
枳实提取物缓解低鱼粉饲料投喂下中华鳖的生长抑制、氧化损伤及肠道炎症
CITRUS AURANTIUM EXTRACT ALLEVIATES GROWTH REDUCTION,OXIDATIVE DAMAGE,AND INTESTINAL INFLAMMATION IN CHINESE SOFT-SHELLED TURTLE(PEODISCUS SINENSIS)FED LOW FISHMEAL DIET
摘要
Abstract
The present study investigated the effects of dietary Citrus aurantium extract(CAE)on growth,immunity,antioxidant capacity,and intestinal health of Chinese soft-shelled turtles(Pelodiscus sinensis)fed low-fishmeal diets.Four experimental diets were formulated:a fishmeal-based control(CON),a low-fishmeal diet(NCON),and NCON supplemented with 0.05%(CAE1)or 0.10%(CAE2)CAE.Compared with NCON,both CAE1 and CAE2 increased weight gain,reduced feed conversion ratio,and improved intestinal villus height and epithelial integrity(P<0.05).Digestive enzyme activities including amylase,lipase,and trypsin were higher in CAE-supplemented groups(P<0.05).Serum ACP,AKP,C3,and C4 levels were elevated,and antioxidant indices(SOD,CAT,GSH,T-AOC)in liver and intestinal increased,with MDA decreased(P<0.05).At the transcriptional level,nrf2,sod,cat,and gpx were upregu-lated in CAE groups,with gpx higher in CAE1 and sod,cat,gpx higher in CAE2(P<0.05).Pro-inflammatory cytokines(nf-κb,mapk,tnf-α,il-1β,il-6,il-8,tlr2)were downregulated,while anti-inflammatory genes(il-10,tgf-β,ppar-α)and tight junction proteins(zo-1,claudin-1,occludin)were upregulated(P<0.05).In conclusion,Citrus aurantium extract improved growth,antioxidant status,and intestinal health in turtles under low-fishmeal diets.关键词
枳实提取物/生长性能/氧化应激/肠道炎症/中华鳖Key words
Citrus aurantium extract/Growth performance/Oxidative stress/Intestinal inflammation/Pelodiscus sinensis分类
农业科技引用本文复制引用
侯尚峰,蔡明浪,乐玉华,张俊智,徐树德,钟蕾,胡毅,罗祖国,戴济鸿..枳实提取物缓解低鱼粉饲料投喂下中华鳖的生长抑制、氧化损伤及肠道炎症[J].水生生物学报,2026,50(6):11-23,13.基金项目
湖南省重点研发计划(2025JK2025) （2025JK2025）
芙蓉计划省企业科技创新创业团队计划支持项目(新瑞生物特种水产饲料研发创新创业团队) （新瑞生物特种水产饲料研发创新创业团队）
湖南省现代农业(水产)产业技术体系项目(HARS-07) （水产）
枳实提取物在水产动物中的应用(xczx-2023158)资助[Supported by the Key Research and Development Program of Hunan Province(2025JK2025) （xczx-2023158）
Hibiscus Plan Provincial Enterprise Technol-ogy Innovation and Entrepreneurship Team Support Program(Xinrui Biological Special Aquatic Feed Research and Development Innovation and Entrepreneurship Team) （Xinrui Biological Special Aquatic Feed Research and Development Innovation and Entrepreneurship Team）
The Earmarked Fund for HARS(HARS-07) （HARS-07）
Application of Citrus aurantium Extract in Aquatic Animals(xczx-2023158)] （xczx-2023158）
