水生生物学报2026，Vol.50Issue(6)：24-33,10.DOI:10.3724/1000-3207.2026.2026.0031
酶解鲍内脏蛋白替代鱼粉对花鲈生长、肝脏抗氧化及肠道健康的影响
DIETARY FISHMEAL REPLACEMENT WITH ABALONE VISCERA HYDROLYZED PROTEIN ON GROWTH PERFORMANCE,HEPATIC ANTIOXIDANT CAPACITY,AND INTESTINAL HEALTH IN SPOTTED SEA BASS(LATEOLABRAX MACULATUS)
摘要
Abstract
To investigate the optimal replacement level of fishmeal with abalone viscera hydrolysate(AVH)in the diet of spotted sea bass(Lateolabrax maculatus)and its effect on fish health,a feeding trial was conducted for 8 weeks.Juvenile spotted sea bass[initial body weight:(141.22±0.38)g]were fed five isonitrogenous and isolipidic diets with AVH replacing 0,10%,20%,30%,and 40%of fishmeal,respectively.Growth performance,serum biochemistry,hepatic antioxidant parameters,intestinal digestion,and gut microbiota(via 16S rRNA sequencing)were analyzed.Results showed that the specific growth rate was the highest in 30%replacement group,with no significant difference compared to the control.In the 40%replacement group,the activities of alanine aminotransferase and aspartate amino-transferase were significantly increased,and hepatic malondialdehyde content was significantly elevated,indicating liver oxidative damage.Serum immune parameters showed that complement C4 content was increased while immunoglobulin M(IgM)level was significantly decreased in the 40%group,suggesting immune dysfunction.Intesti-nal morphology analysis revealed significantly reduced villus height with loose structure,along with significant increases in D-lactate and diamine oxidase levels in the 40%group.Trypsin activity decreased with increasing replace-ment level,whereas lipase and amylase activities showed no significant changes.Gut microbiota analysis indicated that the relative abundance of the genus Cetobacterium first increased and then decreased with increasing AVH inclusion,peaking in the 20%replacement group.This study demonstrates that replacing 30%of fishmeal with AVH does not adversely affect the growth,liver function,or intestinal health in spotted sea bass.However,replacement at 40%leads to liver metabolic disorders,oxidative stress,intestinal structural damage,and impaired digestive function.关键词
鲍内脏/酶解蛋白/鱼粉替代/花鲈/生长性能/肠道健康Key words
Abalone viscera/Enzymatic hydrolysate/Fishmeal replacement/Spotted sea bass/Growth performance/Intestinal health分类
农业科技引用本文复制引用
董俊峰,齐海深,张春晓,王玲,宋凯,李学山,马瑞娟,鲁康乐..酶解鲍内脏蛋白替代鱼粉对花鲈生长、肝脏抗氧化及肠道健康的影响[J].水生生物学报,2026,50(6):24-33,10.基金项目
国家重点研发计划"低粮低鱼粉鱼油水产饲料高效利用技术与示范应用"(2024YFD2402001) （2024YFD2402001）
福建省促进海洋与渔业产业高质量发展专项资金(FJHYF-L-2023-6)资助[Supported by the National Key R&D Program of China(2024YFD2402001) （FJHYF-L-2023-6）
The Special Fund Project for Promoting High-Quality Development of Marine and Fishery Industry of Fujian Province(FJHYF-L-2023-6)] （FJHYF-L-2023-6）
