水生生物学报2026，Vol.50Issue(6)：53-62,10.DOI:10.3724/1000-3207.2026.2026.0049
甲壳动物中白斑综合征病毒来源小RNA的特征及其保守富集的结构基础
CHARACTERISTICS OF WHITE SPOT SYNDROME VIRUS-DERIVED SMALL RNAS IN CRUSTACEANS AND THE STRUCTURAL BASIS OF THEIR CONSERVED ENRICHMENT
摘要
Abstract
To systematically investigate the characteristics of white spot syndrome virus(WSSV)-derived small inter-fering RNAs(vsiRNAs)in different crustacean hosts,publicly available small RNA and miRNA sequencing datasets of crustaceans were retrieved and analyzed from the NCBI SRA database.After data preprocessing,all reads were strin-gently mapped to the WSSV reference genome using a zero-mismatch strategy.Based on the mapping results,five representative crustacean hosts,Penaeus vannamei,P.penicillatus,Scylla paramamosain,Carcinus maenas,and Procambarus clarkii,were selected for analysis of WSSV-derived vsiRNA characteristics.The results showed that abundant WSSV-derived vsiRNAs were detected in P.vannamei and C.maenas,with a predominant length of 22 nt and distribution on both the positive and negative strands of the viral genome,exhibiting typical features of small inter-fering RNAs.In contrast,the other three hosts displayed much lower amounts of WSSV-derived vsiRNAs with dispersed length distributions and no obvious dominant peak.Although overall distribution patterns of WSSV-derived vsiRNAs varied among hosts,shared genomic regions of WSSV consistently exhibited vsiRNA enrichment across multiple species.Sequence and structural features of these hotspot regions,including elevated GC content,lower mini-mum free energy,and enrichment of conserved motifs forming double-stranded or hairpin structures,suggest that RNA secondary structure may contribute to the preferential processing of these regions by the RNAi pathway.This study provides a comparative overview of WSSV-derived vsiRNA characteristics in five crustacean species based on public sequencing data,and offers insights into crustacean RNAi responses to WSSV infection and a reference for future stu-dies on antiviral mechanisms and RNAi-based disease control strategies.关键词
白斑综合征病毒/甲壳动物/RNA干扰/病毒来源小干扰RNAsKey words
White spot syndrome virus/Crustaceans/RNA interference/Virus-derived small interfering RNAs分类
农业科技引用本文复制引用
亓玉华,何嘉乐,刘萍萍,高杰,孙磊,王显伟..甲壳动物中白斑综合征病毒来源小RNA的特征及其保守富集的结构基础[J].水生生物学报,2026,50(6):53-62,10.基金项目
国家自然科学基金(32525058)资助[Supported by the National Natural Science Foundation of China(32525058)] （32525058）
