水生生物学报2026，Vol.50Issue(6)：63-72,10.DOI:10.3724/1000-3207.2025.2025.0437
鱼源性嗜酸乳杆菌AC对斑马鱼幼鱼氨氮抗性的影响
FISH-DERIVED LACTOBACILLUS ACIDOPHILUS AC ON AMMONIA NITRO-GEN RESISTANCE OF JUVENILE ZEBRAFISH(DANIO RERIO)
摘要
Abstract
To investigate the protective effects of fish-derived Lactobacillus acidophilus AC on juvenile zebrafish(Danio rerio)under ammonia nitrogen stress,a total of 180 six-week-old AB strain juvenile Danio rerio[mean weight(83.02±4.08)mg]were randomly divided into four groups:AC0 group(0 CFU/mL Lactobacillus acidophilus AC),AC5 group(105 CFU/mL),AC6 group(106 CFU/mL),and AC7 group(107 CFU/mL).The bacteria were added to the rearing water for 32d,after which fish were exposed to 35.80 mg/L total ammonia nitrogen for 48h.The gill antioxi-dant parameters,gill tissue pathological changes,and the expression of related genes in gill and brain tissues were examined.Antioxidant parameters analysis of the gill tissues revealed that the activity of Na+/K+-ATPase and total superoxide dismutase(SOD)was significantly higher in the AC7 group than that in the AC0(P<0.05),while the malon-dialdehyde(MDA)content was significantly lower(P<0.05).Pathological analysis of the gill tissues showed varying degrees of damage across groups.The AC0 group exhibited severe gill damage,characterized by curling of the gill fila-ments and noticeable edema,degeneration,and necrosis of pillar epithelial cells at the base of lamellae.In contrast,the experimental groups exhibited milder gill damage.Gene expression analysis showed that inflammatory cytokine genes were generally downregulated in treated fish compared to the control group,with the brain factor-α(TNF-α)expression in AC7 significantly lower than that in the AC0(P<0.05).In gill tissue,expression of glutamate dehydrogenase(GDH)and SOD was significantly higher in AC7 than that in AC0 and AC5(P<0.05),while glutamine synthetase(GS)expres-sion in both gill and brain was significantly higher in AC7 than that in the AC0 and AC5(P<0.05).Additionally,B-cell lymphoma-2(Bcl-2)expression in gill tissues was significantly upregulated in AC7 relative to other groups(P<0.05),and caspase 8 expression in gill and brain tissues was downregulated to varying degrees in all treated groups.In conclu-sion,Lactobacillus acidophilus AC alleviates oxidative stress damage in the gills by regulating the expression of antioxidant and ammonia assimilating enzyme genes,thus helping juvenile Danio rerio resist ammonia nitrogen stress.关键词
嗜酸乳杆菌AC/氨氮胁迫/抗氧化酶/鳃组织调控/基因表达调控/斑马鱼Key words
Lactobacillus acidophilus AC/Ammonia nitrogen stress/Antioxidant enzymes/Gill tissue damage/Regu-lation of gene expression/Danio rerio分类
农业科技引用本文复制引用
许嘉航,邱浩宇,冉志强,黄林峰,王闪闪,陶晨智,王萍..鱼源性嗜酸乳杆菌AC对斑马鱼幼鱼氨氮抗性的影响[J].水生生物学报,2026,50(6):63-72,10.基金项目
国家自然科学基金(42176119)资助[Supported by the National Natural Science Foundation of China(42176119)] （42176119）
