水生生物学报2026，Vol.50Issue(6)：124-134,11.DOI:10.3724/1000-3207.2026.2026.0043
西北干旱区一级保护植物发菜的分子多样性及念珠藻属分类学研究
MOLECULAR DIVERSITY OF THE GRADE Ⅰ PROTECTED PLANT NOSTOC FLAGELLIFORME(FAT CHOY)AND TAXONOMY STUDY OF THE GENUS NOSTOC IN THE ARID REGION OF NORTHWEST CHINA
摘要
Abstract
In this study,wild samples of Nostoc flagelliforme were collected across five geographical units,namely Inner Mongolia,Ningxia,Qinghai,Gansu,and Xinjiang.A polyphasic taxonomic and molecular diversity investigation was conducted by integrating morphological observation,16S rRNA gene sequence analysis,and 16S—23S ITS secondary structure analysis.Phylogenetic trees based on the 16S rRNA gene revealed that N.flagelliforme did not form an independent,highly supported monophyletic clade,but was scattered across multiple evolutionary lineages.16S rRNA gene similarity among different samples ranged from 98.4%to 99.7%.However,significant intraspecific differentiation was observed in the D1—D1'and V3 helices,which can serve as precise molecular identification mar-kers.This study confirms the existence of a conflict between the current morphological species definition and molecu-lar evidence,revealing that N.flagelliforme is not a natural monophyletic group and that cryptic species are highly probable.The molecular diversity pattern of N.flagelliforme in Northwest China revealed in this study not only clari-fies its phylogenetic position,but also provides a standardized molecular identification ruler,offering scientific support for wild population resource surveys and germplasm resource bank construction.关键词
分子多样性/西北干旱区/资源保护/发菜Key words
Molecular diversity/Arid northwest region of China/Resource conservation/Nostoc flagelliforme分类
农业科技引用本文复制引用
席亚丽,蔡芳芳,刘玉婷,魏生龙,马彪,刘彦萍,程耀,李仁辉..西北干旱区一级保护植物发菜的分子多样性及念珠藻属分类学研究[J].水生生物学报,2026,50(6):124-134,11.基金项目
2025年度省级科技计划基础研究计划自然科学基金(25JRRG040) （25JRRG040）
2023年张掖市揭榜挂帅项目(ZY2023JBGS09)资助[Supported by the Natural Science Foundation Project of Basic Research Program,Provincial Science and Technology Plan 2025(25JRRG040) （ZY2023JBGS09）
Key Project via Open Tender of Zhangye City 2023(ZY2023JBGS09)] （ZY2023JBGS09）
