生物安全学报（中英文）2026，Vol.35Issue(2)：118-125,8.DOI:10.3969/j.issn.2095-1787.20250070
马铃薯甲虫气味受体蛋白OR30的功能鉴定
Functional characterization of the odorant receptor protein OR30 in Leptinotarsa decemlineata
摘要
Abstract
[Aim]This study aims to examine the function of LdecOR30 in the Colorado potato beetle,Leptinotarsa decemlineata.To do so,the odorant receptor gene OR30 from the antennae was cloned to determine the expression levels of LdecOR30 in different tis-sue samples of male and female adults,and investigate its electrophysiological responses to the main alcohols in various odor mole-cules.[Method]On the basis of the full-length transcriptome and next-generation transcriptome re-sequencing data of L.decemlin-eata,the coding sequence(CDS)of LdecOR30 was cloned by PCR.Quantitative real-time PCR(qPCR)was employed to analyze the expression profiles of LdecOR30 in the antennae,head(without antennae),thorax,abdomen,legs,and wings.The electrophys-iological responses of LdecOR30 to 16 alcohols were examined via the Drosophila empty neuron system combined with single sensil-lum recording.A Y-tube olfactometer was employed to test the effects of specifically responsive compounds on the behavior of L.de-cemlineata.[Result]The full-length cDNA sequence of LdecOR30 was successfully cloned.Sequence analysis indicated that this o-dorant receptor contained six transmembrane domains.qPCR results showed significantly higher expression of LdecOR30 in the anten-nae of both male and female adults than in other tissue samples.Single sensillum recordings in the Drosophila empty neuron system indicated that LdecOR30 responded to five alcohols,with an increase of over 20 spikes per second.Y-tube olfactometer assays dem-onstrated that cis-3-hexen-1-ol had a significant attractive effect on female beetles,with an attraction rate of 80％,while 1-octen-3-ol exhibited significant repellency of 70％to both male and female adults.[Conclusion]The odorant receptor gene LdecOR30 is highly expressed in the antennae of L.decemlineata adults,regardless of sex,and responds to five different alcohols,including cis-3-hexen-1-ol.These findings suggest that LdecOR30 plays an important role in the recognition and localization of host plants by L.de-cemlineata.关键词
马铃薯甲虫/气味受体/电生理反应/Y型嗅觉仪/qPCR/单感器记录Key words
Leptinotarsa decemlineata/odorant receptor/electrophysiological responses/Y-tube olfactometer/qPCR/single sensil-lum recording引用本文复制引用
李彤,李红卫,张柳,陈珍珍,于艳雪..马铃薯甲虫气味受体蛋白OR30的功能鉴定[J].生物安全学报（中英文）,2026,35(2):118-125,8.基金项目
北京市自然科学基金资助项目(6234048) （6234048）
海南省重点研发项目(ZDYF2025XDNY09) （ZDYF2025XDNY09）
