生物安全学报（中英文）2026，Vol.35Issue(2)：172-182,11.DOI:10.3969/j.issn.2095-1787.20250090
防治烟草青枯病的生防细菌FY3-17的鉴定及生物学特性
Identification and biological characterization of the biocontrol bacterial strain FY3-17 against tobacco bacterial wilt
摘要
Abstract
[Aim]This study investigated the antibacterial mechanism of the extract from a biocontrol bacterium isolated from tobac-co rhizosphere soil against Ralstonia solanacearum,the causal agent of tobacco bacterial wilt,aiming to provide a new candidate strain for the biocontrol of this disease.[Method]Antagonistic bacteria with significant inhibitory effects against R.solanacearum were isolated from the rhizosphere soil of healthy tobacco plants via the plate confrontation assay.The selected strain was identified through morphological,physiological,biochemical,and molecular biological analyses.Its disease control and plant growth-promo-ting efficacy were evaluated through greenhouse experiments,and its colonization in tobacco rhizosphere soil and tobacco plants was detected via an antibiotic marker method.Furthermore,a 96-well plate method was employed to determine the minimum inhibitory concentration(MIC)of the bacterial extract against R.solanacearum,and thus evaluating the effect of the strain on R.so-lanacearum.[Result]A biocontrol bacterial strain,designated FY3-17,which exhibited significant inhibitory activity against R.so-lanacearum,was isolated from tobacco rhizosphere soil and identified as Serratia marcescens.Greenhouse experiment results showed that the fermentation broth of FY3-17 provided the control efficacy of 73.98％against tobacco bacterial wilt and significantly promoted tobacco plant growth.Furthermore,this strain stably colonized both the tobacco rhizosphere soil and the tobacco plants.On day 42 post-inoculation,the maximum colonization density(2.0×104 CFU•g-1)was observed in rhizosphere soil,followed by roots(5.35×103 CFU•g-1),stems(8.90×102 CFU•g-1),and leaves(4.57×101 CFU•g-1).Additionally,the extract of FY3-17 significantly inhibited the growth of R.solanacearum,with an MIC of 40 μg•mL-1,and it interfered with the biofilm formation and extracellular polysaccharide production.[Conclusion]The S.marcescens strain FY3-17 screened in this study demonstrates considerable potential for controlling tobacco bacterial wilt and promoting plant growth,indicating promising prospects for development and application.关键词
粘质沙雷氏菌FY3-17/青枯雷尔氏菌/烟草青枯病/生物防治Key words
Serratia marcescens FY3-17/Ralstonia solanacearum/tobacco bacterial wilt/biological control引用本文复制引用
李漪梅,王菊香,杨应伟,王金星,姚博,姬广海,孙雁..防治烟草青枯病的生防细菌FY3-17的鉴定及生物学特性[J].生物安全学报（中英文）,2026,35(2):172-182,11.基金项目
景谷烟区烟草青枯病综合防控技术研究与应用(PEYCKY202201) （PEYCKY202201）
