生物安全学报（中英文）2026，Vol.35Issue(2)：183-194,12.DOI:10.3969/j.issn.2095-1787.20250106
诱抗蛋白fTDP复配宁南霉素防治烟草花叶病
Control of tobacco mosaic disease by combined application of the resistance inducer fTDP and ningnanmycin
摘要
Abstract
[Aim]The fusion TEER-decreasing protein(fTDP)induces tobacco resistance to tobacco mosaic virus(TMV).To en-hance the field efficacy and stability of fTDP,we optimized the combination ratios of agents and explored the induction mechanism.[Method]Using Nicotiana tabacum var.Samsun NN and N.tabacum K326 as materials,we analyzed the number of necrotic spots,disease symptoms,and expression levels of the TMV coat protein gene(TMV-CP)in tobacco plants treated with different agents to identify the most effective antiviral agent.The optimal ratio of the selected agent,ningnanmycin,to fTDP was determined via the co-toxicity coefficient method.The disease resistance efficacy and resistance pathways of the fTDP/ningnanmycin combined agent were evaluated based on indicators such as plant growth status,defense enzymes,and defense genes.The field application efficacy of the combined agent was measured with Yunyan 87 as the material.[Result]Ningnanmycin and fTDP delayed the appearance of mosaic symptoms of tobacco and significantly reduced the viral load.When the mass ratio of fTDP to ningnanmycin was 3:7,the combined agent exhibited a significant synergistic effect of TMV inactivation,with a co-toxicity coefficient of 152.When the mass ratio of fTDP to ningnanmycin was 1:9,the combined agent protected tobacco K326 from TMV infection,with a significant synergistic effect.In addition,the combined agent increased salicylic acid content,defense enzyme activity,and expression levels of multiple disease re-sistance-related protein genes in tobacco K326.Field trial results demonstrated that the combined agent at this ratio exhibited higher and more stable efficacy than the single agents,achieving the control efficacy of 77.8％.[Conclusion]The fTDP/ningnanmycin combined agent enhances tobacco resistance to TMV by activating the salicylic acid signaling pathway.When the mass ratio of fTDP to ningnanmycin was 1:9,the combined agent outperformed single agents in both antiviral activity and cost reduction in both labo-ratory and field settings.关键词
烟草花叶病毒/生物农药/复配剂/田间防治效果Key words
tobacco mosaic virus/biological farm chemical/combined agent/field control effect引用本文复制引用
张焕然,王建枫,文旭,漆育婷,涂班武,吴丽萍..诱抗蛋白fTDP复配宁南霉素防治烟草花叶病[J].生物安全学报（中英文）,2026,35(2):183-194,12.基金项目
江西省自然科学基金(20252BAC250143). （20252BAC250143）
